提出一种基于81°倾斜光纤光栅(81°-TFG)的新城疫病毒(NDV)免疫传感器。分析81°-TFG传感器的基本原理和传感特性。利用金黄色葡萄球菌蛋白A(SPA)修饰81°-TFG的表面,然后将自制的高纯度新城疫病毒单克隆抗体(MAbs)通过SPA分子固定于81°-TFG表面,从而制成对NDV抗原特异性检测的传感器。实验结果表明:该免疫传感器对NDV的探测极限在0.1 ~0.2 ng/mL之间,检测饱和点约为1.0 ng/mL;在0~1.0 ng/mL范围内具有良好的线性度(R²约为 0.982),灵敏度约为342 pm/(ng·mL ^-1);且该传感器具有良好的可重用性和对NDV抗原的高度特异性,并能用于NDV的临床检测。相对于传统的免疫荧光技术、酶联免疫等生化检测方法,本文提出的方法具有免标记、操作简便、快速检测等优点。

A type of newcastle disease virus (NDV) immunosensor based on 81° tilted fiber grating (81°-TFG) is proposed. The basic principle and sensing properties of the 81°-TFG are briefly analyzed. Staphylococcus aureus protein A (SPA) is used to modify the surface of 81°-TFG, and then highly purified NDV monoclonal antibodies (MAbs) are immobilized on 81°-TFG surface through SPA molecules. Finally, the NDV immunosensor is successfully developed. The experimental results show that the immunosensor has a lowest limit of detection to NDV of between 0.1 ng/mL and 0.2 ng/mL, gets saturated at about 1.0 ng/mL, and has good linearity with R2 of about 0.982 and sensitivity of about 342 pm/(ng·mL -1) in the concentration range from 0 to 1.0 ng/mL. In addition, this immunosensor shows good reusability and high specificity to NDV, and can also be used in clinical test for NDV. Compared with the traditional biochemical methods such as immunofluorescence technique, enzyme-linked immunoabsorbent assay, this technique proposed in this paper has the advantages of no mark, simple operation, fast detection.