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参麦注射液与人血清白蛋白相互作用的多光谱研究

Multi-spectral analysis of interaction between Shenmai injection and human serum albumin

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摘要

参麦注射液是一种中药复合物, 广泛应用于癌症患者的辅助治疗中。在生理(PH7.4)环境下, 通过荧光光谱与紫外吸收光谱研究了参麦注射液与人血清白蛋白的相互作用。 荧光光谱和紫外吸收光谱实验结果表明, 参麦注射液能够有效地引起人血清白蛋白的内荧光源淬灭, 其淬灭机理为动态淬灭。利用Stern-Volmer方程对荧光光谱数据进行分析, 得到不同温度(296, 303, 310 K)下的结合常数(KA)。通过Van′t Hoff 方程计算出热力学参数(ΔG<0, ΔH>0, ΔS>0), 该结果表明疏水力是人血清白蛋白与参麦注射液结合时的主要相互作用力及结合过程是一个自发过程。此外, 同步荧光光谱实验结果表明, 当参麦注射液与人血清白蛋白结合时, 主要结合点位于酪氨酸残基, 且引起了人血清白蛋白的结构变化。

Abstract

Shenmai injection is compound traditional medicine and widely employed in adjuvant therapy of cancer patients for improving the patient′s life. The interaction of Shenmai injection with human serum albumin(HSA) in physiological buffer(PH 7.4) was investigated by fluorescence spectroscopy and UV-Vis absorption spectroscopy. These results have significant importance for understanding the pharmacological action pesticide effect of Shenmai injection. Shenmai injection can effectively quench the intrinsic fluorescence of HSA and the results shown that the quenching mechanism was a dynamic process, which was further proved by the UV-Vis absorption spectroscopy. The binding constant KA at different temperatures(296, 303, 310 K) were obtained from Modified Stern-Volmer analysis of the fluorescence quenching data. The thermodynamic parameters were calculated by Van′t Hoff equation(ΔG<0, ΔH>0, ΔS>0). It indicated that the hydrophobic interactions play an important role in the interaction of Shenmai injection and HSA. In addition, the binding process was spontaneous. Furthermore, the synchronous fluorescence spectra showed that the maximum fluorescence peak of tyrosine residues changed which meant the binding of Shenmai injection to HSA mainly acting on tyrosine residues and the interaction can induce the microenvironment and conformation changes of HSA.

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中图分类号:O433.4

DOI:10.3788/irla201746.1123001

所属栏目:光谱探测与分析

基金项目:国家自然科学基金(61377001)

收稿日期:2017-03-05

修改稿日期:2017-04-03

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作者单位    点击查看

林晓钢:重庆大学 光电技术及系统教育部重点实验室, 重庆 400044
翁凌冬:重庆大学 光电技术及系统教育部重点实验室, 重庆 400044
朱濠:重庆大学 光电技术及系统教育部重点实验室, 重庆 400044
宛楠:重庆大学 光电技术及系统教育部重点实验室, 重庆 400044
叶长彬:重庆大学 光电技术及系统教育部重点实验室, 重庆 400044
杜基赫:重庆大学 光电技术及系统教育部重点实验室, 重庆 400044

联系人作者:林晓钢(xglin@cqu.edu.com)

备注:林晓钢(1975-), 男, 副教授, 博士, 主要从事生物医学光子方面的研究。

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引用该论文

Lin Xiaogang,Weng Lingdong,Zhu Hao,Wan Nan,Ye Changbin,Du Jihe. Multi-spectral analysis of interaction between Shenmai injection and human serum albumin[J]. Infrared and Laser Engineering, 2017, 46(11): 1123001

林晓钢,翁凌冬,朱濠,宛楠,叶长彬,杜基赫. 参麦注射液与人血清白蛋白相互作用的多光谱研究[J]. 红外与激光工程, 2017, 46(11): 1123001

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