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QuEChERS-拉曼光谱法测定黄瓜上的吡虫啉残留量

QuEChERS-Raman spectroscopy method for detecting imidacloprid residue in cucumbers

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摘要

采用拉曼光谱技术结合QuEChERS(Quick Easy Cheap Effective Rugged and Safe)样本前处理建立了黄瓜上吡虫啉残留量的快速检测方法。以进行了不同前处理步骤(乙腈提取、去水萃取、褪色除杂)的三批黄瓜样本作为实验对象, 利用780 nm激光器采集样本的拉曼光谱图, 并分别采取偏最小二乘(PLS)和主成分回归(PCR)算法建立了六个黄瓜中吡虫啉含量预测模型。结果表明, 仅进行了乙腈(C2H3N)提取一步前处理的样本建模效果最优, 校正集及预测集的相关系数均在0.99以上, 其中PLS的预测集相对分析误差(RPD)达到5.52, 说明模型具有一定的预测精度, 此结果可为后续前处理简化研究提供有力依据。

Abstract

Raman spectroscopy has been more and more frequently used for pesticide residue detection research in recent years, but the development of sample pretreatment technology is relatively lagging behind. In the study, a rapid method had been developed for the determination of imidacloprid residue in cucumbers with the application of Raman spectroscopy technology and Quick Easy Cheap Effective Rugged and Safe(QuEChERS) sample preparation. Three batches of cucumber samples (the concentration of imidacloprid was within the range of 0.2-5 mg/kg) with different preparation steps(acetonitrile extraction, dehydrate extraction, and fading removing impurity) were chosen as experimental objects. Confocal micro Raman spectrometer was utilized with a 780 nm laser to collect three batches of samples of Raman spectra. Six quantitative prediction models of imidacloprid residue were established based on PLS and PCR methods. The results showed that in addition to the PCR model of the samples by two steps preparation, the residual predictive deviation(RPD) of the other five models was higher than 3. The samples which were only extracted with acetonitrile got the best modeling effect. The correlation coefficient of the calibration set and the prediction set were all above 0.99. The root mean square error of prediction(RMSEP) of PLS method was 0.148 mg/kg, and the RPD was 5.52, which obtained the highest precision of the six predictive models. The results could provide a strong basis for the following-up studies.

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中图分类号:S3

DOI:10.3788/irla201746.1123002

所属栏目:光谱探测与分析

基金项目:北京市自然科学基金(4142012); 北京市教委科研计划重点项目(KZ201310011012); 北京市教委科技创新平台(PXM_2012_014213_000023)

收稿日期:2017-03-05

修改稿日期:2017-04-03

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刘翠玲:北京工商大学 计算机与信息工程学院, 北京 100048北京工商大学 计算机与信息工程学院 食品安全大数据技术北京市重点实验室, 北京 100048
赵琦:北京工商大学 计算机与信息工程学院, 北京 100048北京工商大学 计算机与信息工程学院 食品安全大数据技术北京市重点实验室, 北京 100048
孙晓荣:北京工商大学 计算机与信息工程学院, 北京 100048北京工商大学 计算机与信息工程学院 食品安全大数据技术北京市重点实验室, 北京 100048
邢瑞芯:北京工商大学 计算机与信息工程学院, 北京 100048北京工商大学 计算机与信息工程学院 食品安全大数据技术北京市重点实验室, 北京 100048

联系人作者:刘翠玲(liucl@btbu.edu.cn)

备注:刘翠玲(1963-), 女, 教授, 博士, 主要从事智能测量技术与数据处理、系统建模与仿真方法研究、智能控制方法方面的研究。

【1】Lin Jin, Chen Yue. Detection of pesticide residues in vegetables from a university of Shenzhen in 2010[J]. Modern Food Science & Technology, 2011, 27(8): 1012-1014. (in Chinese)

【2】Fan Y, Lai K, Rasco B A, et al. Analyses of phosmet residues in apples with surface-enhanced Raman spectroscopy [J]. Food Control, 2013, 37(1): 153-157.

【3】Shende C, Inscore F, Sengupta A, et al. Rapid extraction and detection of trace Chlorpyrifos-methyl in orange juice by surface-enhanced Raman spectroscopy[J]. Sensing & Instrumentation for Food Quality & Safety, 2010, 4(3):101-107.

【4】Anastassiades M, Lehotay S J, Stajnbaher D, et al. Fast easy multi residue method employing acetonitrile extraction/partitioning, "Dispersive solid-phase extraction" for the determination of pesticide residues in produce[J]. Journal of Aoac International, 2003, 86(2): 412-431.

【5】Lehotay S J. Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study[J]. Journal of Aoac International, 2007, 90(2): 485-520.

【6】Payá P, Anastassiades M, Mack D, et al. Analysis of pesticide residues using the Quick Easy Cheap Effective Rugged and Safe(QuEChERS) pesticide multi residue method in combination with gas and liquid chromatography and tandem mass spectrometric detection [J]. Analytical & Bio Analytical Chemistry, 2007, 389(6): 1697-1714.

【7】Yi Jianghua, Duan Zhengjuan, Fang Guozhen, et al. Study of QuEChERS method for the detection of pesticide and veterinary drug residues in foods[J]. Journal of Chinese Institute of Food Science and Technology, 2013, 13(2):153-158. (in Chinese)

【8】Cherta L, Beltran J, López F, et al. Application of fast gas chromatography-mass spectrometry in combination with the QuEChERS method for the determination of pesticide residues in fruits and vegetables[J]. Food Analytical Methods, 2013, 6(4): 1170-1187.

【9】Lu D, Qiu X, Feng C, et al. Simultaneous determination of 45 pesticides in fruit and vegetable using an improved QuEChERS method and on-line gel permeation chromatography-gas chromatography/mass spectrometer[J]. Journal of Chromatography B Analytical Technologies in the Biomedical & Life Sciences, 2012, 895-896(3): 17-24.

【10】Xie Feng, Sun Haida, Li Zhanbin, et al. QuEChERS sample preparation method for rapid screening of malathion in legume vegetables by surface-enhanced Raman spectroscopy[J]. Food Science and Technology, 2014, 35(8): 286-290. (in Chinese)

【11】Peng Y, Dhakal S, Chao K, et al. Research on identification and determination of mixed pesticides in apples using surface enhanced Raman spectroscopy[C]//SPIE Sensing Technology and Applications, 2015, 9488: 94880R.

【12】Huang S, Hu J, Guo P, et al. Rapid detection of chlorpyriphos residue in rice by surface-enhanced Raman scattering [J]. Analytical Methods, 2015, 7(10): 4334-4339.

【13】Norris K H, Williams P C. Optimization of mathematical treatments of raw near-infrared signal in the measurement of protein in hard red spring wheat, I: Influence of particle size [J]. Cereal Chemistry, 1984, 61(2): 158-165.

【14】Chu Xiaoli. Molecular Spectroscopy Analytical Technology Combined with Chemometrics and Its Applications[M]. Beijing: Chemical Industry Press, 2011. (in Chinese)

【15】Fang Xiaorong, Zhang Hailiang, Huang Lingxia, et al. Measurement of soil total nitrogen using near infrared spectroscopy combined with RCA and SPA[J]. Spectroscopy and Spectral Analysis, 2015, 35(5): 1248-1252. (in Chinese)

引用该论文

Liu Cuiling,Zhao Qi,Sun Xiaorong,Xing Ruixin. QuEChERS-Raman spectroscopy method for detecting imidacloprid residue in cucumbers[J]. Infrared and Laser Engineering, 2017, 46(11): 1123002

刘翠玲,赵琦,孙晓荣,邢瑞芯. QuEChERS-拉曼光谱法测定黄瓜上的吡虫啉残留量[J]. 红外与激光工程, 2017, 46(11): 1123002

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