建立FOS-μSIA-LOV光谱在线过程分析, 实现自动化含量测定头孢拉定(RAD)。 通过程序编写, 元件智能控制, 优化实验条件, 考察了RAD中的辅料, 载液, 进样体积等因素。 优选条件为: 载液吸入体积为500 μL; RAD吸入体积为100 μL; 打出体积的流速为150 μL·s-1, RAD质量浓度c与ΔF的线性关系为ΔF=812.6c+284（0.05～0.5mg·mL-1, r=0.999 5）, RSD为4.4%, 回收率为101%～103%。 通过FOS-μSIA-LOV系统和设定特定检测程序, 实现自动化在线过程分析, 具有重现性好、 节约试剂、 省时省力的优点。

The content of cephradine capsules was determined by a system of FOS-μSIA-LOV(fiber optic sensor-micro sequential injection-valve laboratory), based on the principle of cefradine quenching fluorescein in methanol solvent, optimize experimental conditions. The order of inhalation was anhydrous methanol, cefradine, fluorescein and anhydrous methanol; the carrier liquid suction volume was 500 μL; cefradine inhalation volume was 100 μL. Cefradine quenched fluorescein fluorescence intensity and concentration in 0.05～0.5 mg·mL-1 linear relationship as ΔF=812.6+284.41, r=0.999 5, precision RSD was 4.4%, the recovery was 101%～103%. FOS-SIA-LOV and personalized drug testing and programming resulted in automated on-line process analysis with the good reproducibility, less reagent consumption, time and labor saving.