采用衰减全反射红外光谱(ATR-FTIR)技术,研究了固相葡萄糖的结构。实验发现: 固相葡萄糖同时存在着O-H伸缩振动模式(νOH),CH2 不对称伸缩振动模式(νasCH2),CH2 对称伸缩振动模式(νsCH2),CH2 变角振动模式(δCH2),C-O伸缩振动模式(νC-O),O-H面内弯曲振动模式(δOH),葡萄糖环振动模式(νⅠ)和葡萄糖环呼吸振动模式(νⅢ)等。采用二维红外光谱(2D-IR)技术,研究了固相葡萄糖νⅠ和νⅢ。实验发现: 固相葡萄糖存在着 α-D-(+)-吡喃葡萄糖及 β-D-(+)-吡喃葡萄糖两种异构体,并进一步来研究温度变化对于固相葡萄糖异构体结构的影响。

The solid phase glucose structure had been studied by fourier transform attenuated total reflection infrared spectroscopy(ATR-FTIR).The O-H stretch vibration(νOH),CH2 asymmetrical stretching vibration(νasCH2),CH2symmetrical stretching vibration(νsCH2),CH2 scissoring vibration(δCH2),C-O stretch vibration(νC-O),O-H in-plane bending vibration(δOH),glucose skeleton vibration mode(νⅠ) and glucose skeleton breathing vibration(νⅢ) were found.The solid phase glucoses νⅠ and νⅢhad been discussed by two-dimensional infrared spectroscopy(2D-IR).The α-D-glucopyranose and β-D-glucopyranose were discoverd and influence of temperature change on solid glucose structure was researched by 2D-IR also.