中国激光, 2018, 45 (2): 0207018, 网络出版: 2018-02-28   

无透镜显微成像技术在即时检测中的应用进展 下载: 2038次特邀综述

Application Progress of Lensless Microscopy Imaging Technology for Point-of-Care Testing
作者单位
1 西安交通大学生命科学与技术学院生物信息工程教育部重点实验室, 陕西 西安 710049
2 西安交通大学仿生工程与生物力学中心, 陕西 西安 710049
3 西安交通大学理学院化学系, 陕西 西安 710049
摘要
疾病诊断、食品安全监测和环境污染检测等领域常涉及细菌等微生物的即时检测,光学显微镜是常用于检测和分析这些微小样品的工具。无透镜显微成像技术是将样品与电荷耦合元件(CCD)或互补金属半导体氧化物(CMOS)芯片等光检测器紧密接触、无需光学元件、直接对样品进行成像的技术,较传统显微装置具有结构简单、体积小巧、操作简便、价格低廉等优点,已被应用于微小组织结构检查、细胞形态数量分析、微生物检测等领域。根据成像原理,无透镜显微成像技术可分为阴影成像、荧光成像及数字全息成像三类。分别阐述了三种无透镜显微成像技术的成像原理和物理结构,并综述了无透镜显微成像技术在即时检测中的应用,最后展望了无透镜显微成像技术的发展。
Abstract
Microorganisms, such as bacteria, are always detection objects in point-of-care testing, such as infectious diseases diagnosis, food safety monitoring and environment pollution detection. Optical microscopy is always a tool which is used to detect and analyze these detection objects. Lensless microscopy combines the sample with photodetectors such as charge coupled device (CCD) or complementary metal-oxide semiconductor (CMOS). This technique does not need optical devices, and the samples are imaged directly. The device is significantly simpler, smaller, easier to manipulate and cheaper than that of lens-based conventional microscope, which has been used in microstructure examinations, cell morphological analysis and microorganism detection. The lensless microscopic technology can be divided into three types, such as shadow imaging, fluorescence imaging and digital inline holography according to imaging principle. This paper reviews imaging principle and physical structure of three kinds of lensless microscopic technologies, and summaries the applications of lensless microscopic technologies in point-of-care testing. At last, we discuss the future development of lensless microscopic technologies.

李聪慧, 曹若凡, 许夏瑜, 李菲, 张镇西, 徐峰. 无透镜显微成像技术在即时检测中的应用进展[J]. 中国激光, 2018, 45(2): 0207018. Li Conghui, Cao Ruofan, Xu Xiayu, Li Fei, Zhang Zhenxi, Xu Feng. Application Progress of Lensless Microscopy Imaging Technology for Point-of-Care Testing[J]. Chinese Journal of Lasers, 2018, 45(2): 0207018.

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