表面增强拉曼散射(SERS)光谱具有高灵敏度、高分辨率的优点，可表征分子的指纹振动光谱，实现对样品的无损测量，在光谱分析领域具有重要应用。SERS光谱的灵敏度、稳定性及可重复性与所用的SERS基底性能密切相关。目前常用的SERS硬质基底存在制备过程复杂、柔性差、不便携带且易碎的缺点。利用纸张作为支撑的SERS纸质基底能有效解决硬质基底存在的问题，满足未来分析检测领域快速、便携以及个性化的需求。介绍了当前制备纸质SERS基底的主要方法，包括直接浸泡/滴加法、喷墨打印法、化学反应法和物理喷镀法，并对纸质SERS基底在生物医学分析与传感、环境分析以及食品安全等领域的具体应用进展进行了阐述。

Surface-enhanced Raman scattering (SERS) spectroscopy, a technique with high sensitivity and high resolution, can provide the fingerprint vibration spectrum of the analyzed molecule and realize the nondestructive measurement of the sample, which has very important applications in field of spectrum analysis. The sensitivity, stability, and reproducibility of SERS spectrum depend on the performance of SERS substrate. The common SERS rigid substrates have the defects of complex preparation process, poor flexibility, inconvenience to carry, and fragility. Paper-based SERS substrate, which uses paper as support material, can effectively overcome the shortcomings of rigid substrates and meet the commands of fast, handy, and personized analysis and measurement in the future. In this review, we summarize the main methods (direct dipping/dropping, inkjet printing, chemical reaction, and physical spray coating) for the preparation of paper-based SERS substrates, and discuss the applications in biomedical analysis and sensing, environmental monitoring, and food safety.