首页 > 论文 > 激光与光电子学进展 > 55卷 > 5期(pp:50003--1)

线扫描共聚焦成像技术在生物医学成像中的应用

Application of Confocal Line Scanning Imaging Technique in Biomedical Imaging

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

线扫描共聚焦成像技术基于共聚焦成像原理,使用线光束一维扫描照明样品以提高成像速率;通过共焦狭缝滤除样品成像光束中的非聚焦层面杂散光,提高成像分辨率和对比度;近年来,该技术因分辨率高、成像快、成像视场大、系统结构简单等优点而在生物医学成像中的应用越来越广泛。介绍了线扫描共聚焦成像技术的基本原理,列举了成像系统的主要参数及其影响因素,并举例说明了其在生物医学成像,尤其是眼底成像和生物组织细胞观察等方面的应用,最后总结了该技术的优缺点及其应用前景。

Abstract

Based on confocal imaging principle, the confocal line scanning imaging technique uses the one-dimensional scanning-line beam to illuminate the sample for increasing imaging speed. A confocal slit is used to filter the stray light from unfocused-level for improving imaging resolution and contrast ratio. Recently, the confocal line scanning imaging technology is becoming more and more popular in biomedical imaging because of its advantages such as high imaging speed, high imaging resolution, simple system structure, and so on. We introduce the basic principle of confocal line scanning imaging, and list main parameters of the imaging system and their influencing factors. Then the application in biomedical imaging especially in fundus imaging and biological tissue cell observation is illustrated. Advantages, disadvantages, and application prospect of the technique are finally summarized.

Newport宣传-MKS新实验室计划
补充资料

中图分类号:O439

DOI:10.3788/lop55.050003

所属栏目:综述

基金项目:国家自然科学基金(61675226,61378090)、国家重大科学仪器设备开发专项(2016YFF0102000)、中国科学院战略性先导科技专项(XDB02060000)、中国科学院前沿科学重点研究项目(QYZDB-SSW-JSC03)、江苏省杰出青年基金(BK20060010)

收稿日期:2017-10-02

修改稿日期:2017-11-22

网络出版日期:--

作者单位    点击查看

孔文:中国科学院苏州生物医学工程技术研究所, 江苏省医用光学重点实验室, 江苏 苏州 215163
高峰:中国科学院苏州生物医学工程技术研究所, 江苏省医用光学重点实验室, 江苏 苏州 215163
樊金宇:中国科学院苏州生物医学工程技术研究所, 江苏省医用光学重点实验室, 江苏 苏州 215163
史国华:中国科学院苏州生物医学工程技术研究所, 江苏省医用光学重点实验室, 江苏 苏州 215163

联系人作者:史国华(ioe_eye@126.com)

备注:孔文(1992—),男,硕士研究生,主要从事共聚焦在体成像方面的研究。E-mail: kongwen20@163.com

【1】Minsky M. Microscopy apparatus: US3013467[P]. 1961-12-19.

【2】Liang R G.Biomedical optical imaging technologies: design and applications[M]. Berlin: Springer-Verlag, 2012.

【3】Brown C M, Dalal R B, Hebert B, et al. Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope[J]. Journal of Microscopy, 2008, 229(1): 78-91.

【4】He Y, Shi G H, Lu J, et al. High-speed line scanning confocal laser ophthalmoscope[J]. Acta Optica Sinica, 2012, 32(1): 0117001.
何益, 史国华, 卢婧, 等. 高速线扫描共焦检眼镜[J]. 光学学报, 2012, 32(1): 0117001.

【5】Hammer D X, Ferguson R D, Magill J C, et al. Image stabilization for scanning laser ophthalmoscopy[J]. Optics Express, 2002, 10(26): 1542-1549.

【6】Wolleschensky R, Zimmermann B, Kempe M.High-speed confocal fluorescence imaging with a novel line scanning microscope[J]. Journal of Biomedical Optics, 2006, 11(6): 064011.

【7】Mei E, Fomitchov P A, Graves R, et al. A line scanning confocal fluorescent microscope using a CMOS rolling shutter as an adjustable aperture[J]. Journal of Microscopy, 2012, 247(3): 269-276.

【8】Kong W, Lang T T, Gao F, et al. Design of high-resolution wide field of view confocal line scanning laser microscopy[J]. Opto-Electronic Engineering, 2017, 44(6): 616-620.
孔文, 郎婷婷, 高峰, 等. 高分辨率大视场线扫描共焦显微镜的设计与研制[J]. 光电工程, 2017, 44(6): 616-620.

【9】Gareau D S, Abeytunge S, Rajadhyaksha M.Line-scanning reflectance confocal microscopy of human skin: comparison of full-pupil and divided-pupil configurations[J]. Optics Letters, 2009, 34(20): 3235-3237.

【10】Gareau D S, Rajadhyaksha M, Abeytunge S.Engineering of a line-scanning confocal microscope toward imaging epithelial tissues[C]∥Proceedings of Conference on Biomedical Optics. [S.l.]:[s.n.], 2008: BTuF66.

【11】Wang D, Meza D, Wang Y, et al. Sheet-scanned dual-axis confocal microscopy using Richardson-Lucy deconvolution[J]. Optics Letters, 2014, 39(18): 5431-5434.

【12】Simon B, DiMarzio C A. Simulation of a theta line-scanning confocal microscope[J]. Journal of Biomedical Optics, 2007, 12(6): 064020.

【13】Im K B, Han S, Park H, et al. Simple high-speed confocal line-scanning microscope[J]. Optics Express, 2005, 13(13): 5151-5156.

【14】Yin C, Glaser A K, Leigh S Y, et al. Miniature in vivo MEMS-based line-scanned dual-axis confocal microscope for point-of-care pathology[J]. Biomedical Optics Express, 2016, 7(2): 251-263.

【15】He Y. Human retina motion measurement with high speed line scanning confocal fundus imaging system[D]. Chengdu: University of Chinese Academy of Sciences, 2013
何益. 高速线扫描共焦眼底成像技术及其在人眼视网膜抖动检测中的应用研究[D]. 成都: 中国科学院大学, 2013.

【16】Liew G, Wang J J, Cheung N, et al. The retinal vasculature as a fractal: methodology, reliability, and relationship to blood pressure[J]. Ophthalmology, 2008, 115(11): 1951-1956.

【17】Wong T Y, McIntosh R.Systemic associations of retinal microvascular signs: a review of recent population-based studies[J]. Ophthalmic and Physiological Optics, 2005, 25(3): 195-204.

【18】Edwards M S, Wilson D B, Craven T E, et al. Associations between retinal microvascular abnormalities and declining renal function in the elderly population: the cardiovascular health study[J]. American Journal of Kidney Diseases, 2005, 46(2): 214-224.

【19】Hammer D X, Ferguson R D, Ustun T E, et al. Line-scanning laser ophthalmoscope[J]. Journal of Biomedical Optics, 2006, 11(4): 041126.

【20】Dwyer P J, DiMarzio C A, Rajadhyaksha M. Confocal theta line-scanning microscope for imaging human tissues in vivo[J]. Optics and Photonics News, 2006, 17(12): 32.

【21】Dwyer P J,DiMarzio C A, Zavislan J M, et al. Confocal reflectance theta line scanning microscope for imaging human skin in vivo[J]. Optics Letters, 2006, 31(7): 942-944.

【22】Chen Y, Wang D N, Khan A, et al. Video-rate in vivo fluorescence imaging with a line-scanned dual-axis confocal microscope[J]. Journal of Biomedical Optics, 2015, 20(10): 106011.

【23】Iftimia N V, Hammer D X, Bigelow C E, et al. Hybrid retinal imager using line-scanning laser ophthalmoscopy and spectral domain optical coherence tomography[J]. Optics Express, 2006, 14(26): 12909-12914.

【24】Mujat M, Ferguson R D, Iftimia N, et al. Compact adaptive optics line scanning ophthalmoscope[J]. Optics Express, 2009, 17(12): 10242-10258.

【25】Lu J, Gu B Y, Wang X L, et al. High-speed adaptive optics line scan confocal retinal imaging for human eye[J]. PLoS One, 2017, 12(3): e0169358.

【26】Vienola K V, Damodaran M, Braaf B, et al. Parallel line scanning ophthalmoscope for retinal imaging[J]. Optics Letters, 2015, 40(22): 5335-5338.

【27】Vakili A, Xiong D X, Rajadhyaksha M, et al. Line-scanning confocal microscope utilizing high brightness LED[C]// Proceedings of 40th Annual Northeast Bioengineering Conference.[S.l.]: [s.n.], 2014: 1-2.

【28】Carucci J A, Stevenson M, Gareau D. Line-scanning, stage scanning confocal microscope[C]. SPIE, 2016: 97030F.

引用该论文

Kong Wen,Gao Feng,Fan Jinyu,Shi Guohua. Application of Confocal Line Scanning Imaging Technique in Biomedical Imaging[J]. Laser & Optoelectronics Progress, 2018, 55(5): 050003

孔文,高峰,樊金宇,史国华. 线扫描共聚焦成像技术在生物医学成像中的应用[J]. 激光与光电子学进展, 2018, 55(5): 050003

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF