光谱学与光谱分析, 2018, 38 (4): 1319, 网络出版: 2018-06-12
Detecting H2S Gas Concentration by 1,8-Naphthalimides Fluorescent Probe
Detecting H2S Gas Concentration by 1,8-Naphthalimides Fluorescent Probe
光学测量 硫化氢浓度 8-萘酰亚胺衍生物 荧光光谱 Optical measurement Hydrogen sulfide concentration 1 1 8-naphthalimides Fluorescence spectrum
摘要
针对传统硫化氢检测方法灵敏度低的问题, 以1,8-萘酰亚胺为荧光基团, 基于H2S的还原性, 通过在荧光分子结构上引入具有氧化性的硝基, 合成一种可与硫化氢气体发生氧化还原反应生成有荧光响应的小分子荧光探针。 该探针本身荧光十分微弱, 且荧光峰值在λ=467 nm和λ=522 nm处。 与H2S反应之后, 522 nm处的荧光效应消失, 467 nm处的荧光效应显著增强。 测定小分子荧光探针在通入H2S气体前后的荧光光谱, 分析467 nm处的荧光强度与气体浓度关系。 结果表明: 荧光光谱法检测出的H2S气体浓度与荧光强度之间存在很强的线性关系, 相关系数为0.979 3, 最低可检测极限可达0.88×10-6 mol·L-1量级。 表明基于1,8-萘酰亚胺衍生物的荧光光谱检测法可为油气田H2S气体浓度的的快速测定提供参考。
Abstract
Aiming at the low sensitivity of traditional hydrogen sulfide detection method, a small-molecule fluorescent probe was designed and synthesized for hydrogen sulfide detection via introducing oxidizing group of nitro on the 1,8-naphthalimide fluorescence group, based on the nitro group could be reduced by hydrogen sulfide to produce the corresponding amino group. The fluorescence intensity of probe was very weak, and the fluorescence peaks were at λ=467 nm and λ=522 nm. After reacting with H2S, fluorescent effect disappeared at 522 nm, it significantly enhanced at 467 nm. The fluorescence spectrum of fluorescent probe was measured after being introduced into H2S, and the fluorescence intensity at 467 nm was analyzed. The experimental result showed an excellent linear relationship between H2S gas concentration and fluorescence intensity, while the linear correlation coefficient was up to 0.979 3. Meanwhile, the minimum H2S gas concentration that could be detected was only 0.88×10-6 mol·L-1. Fluorescence spectrometric detection of 1,8-naphthalimides solution can be used for the H2S gas rapid determination in oil and gas fields.
唐东林, 王瞧, 出毅能, 李瑞海. Detecting H2S Gas Concentration by 1,8-Naphthalimides Fluorescent Probe[J]. 光谱学与光谱分析, 2018, 38(4): 1319. TANG Dong-lin, WANG Qiao, CHU Yi-neng, LI Rui-hai. Detecting H2S Gas Concentration by 1,8-Naphthalimides Fluorescent Probe[J]. Spectroscopy and Spectral Analysis, 2018, 38(4): 1319.