During the past decades, atomically thin, twodimensional (2D) layered materials have attracted tremendous research interest on both fundamental properties and practical applications because of their extraordinary mechanical, thermal, electrical and optical properties, which are distinct from their counterparts in the bulk format. Various fabrication methods, such as soft-lithography, screen-printing, colloidal-templating and chemical/ dry etching have been developed to fabricate micro/ nanostructures in 2D materials. Direct laser fabrication with the advantages of unique three-dimensional (3D) processing capability, arbitrary-shape designability and high fabrication accuracy up to tens of nanometers, which is far beyond the optical diffraction limit, has been widely studied and applied in the fabrication of various micro/ nanostructures of 2D materials for functional devices. This timely review summarizes the laser-matter interaction on 2D materials and the significant advances on laser-assisted 2D materials fabrication toward diverse functional photonics, optoelectronics, and electrochemical energy storage devices. The perspectives and challenges in designing and improving laser fabricated 2D materials devices are discussed as well.