以巯基丙酸为稳定剂制备了水溶性CdSe/ZnS量子点, 并采用滴涂法制备了CdSe/ZnS修饰的金电极(CdSe/ZnS/GE), 研究其电化学发光(ECL)性质, 考察了pH、CdSe/ZnS浓度、扫描速度、静置时间等实验条件对ECL强度的影响。结果表明, 在碱性溶液中, 去甲肾上腺素(NE)在鲁米诺溶液中对CdSe/ZnS的电化学发光信号有明显的增敏作用, 由此建立了一种检测去甲肾上腺素的新方法。当去甲肾上腺素的浓度(CNE)在2.3×10-5 ~ 1.0×10-8 mol/L范围内时, 去甲肾上腺素的浓度与相对电化学发光强度呈现良好的线性关系。线性回归方程为ΔIECL=118.788CNE－15.333(R=0.994 4), 最低检测限(S/N=3)为0.33×10-8 mol/L。

Mercaptopropionic acid capped CdSe/ZnS quantum dots(QDs) were synthesized in aqueous solution. The CdSe/ZnS QDs modified gold electrode was prepared. The electrochemical luminescence of this modified electrode was studied. Several experiment factors, such as pH of buffer solution, the concentration of CdSe/ZnS QDs, scan rate and rest time were selected. The results show that the CdSe/ZnS modified electrode has an obvious ECL signal of luminol systems in alkaline media, and norepinephrine can enhance electrochemical luminescence(ECL) signal of the luminol system. Based on these, a novel electrochemical luminescence method for the determination of norepinephrine was developed. Under the optimum conditions, ECL intensities had good linearity with the concentration of norepinephrine and the linear range was widely extended from 2.3×10-5 － 1.0×10-8 mol/L. The linear regression equation is ΔIECL=118.788CNE－15.333, and the correlation coefficient was 0.994 4. The detection limit is 0.33×10-8 mol/L.