光子学报, 2018, 47 (6): 0630001, 网络出版: 2018-09-07   

激光诱导植物叶绿素荧光寿命及光谱特性分析

Laser-induced Plant Chlorophyll Fluorescence Lifetime and Spectral Properties Analysis
作者单位
西安工业大学 光电工程学院, 西安 710021
摘要
传统荧光光谱分析法虽能用于评估植物生理状况, 但在植物遥感探测中该方法具有抗干扰性弱、待测数据量大的缺陷.针对此问题, 提出一种荧光寿命成像技术, 将植物荧光寿命及光谱信息结合, 分析植物内在生理信息, 实现了远距离、大范围精确测量植物生理状况.采用时间分辨测量法, 用连续激光脉冲照射植物产生大量相同的荧光信号, 同时不断改变探测器的启动延迟时间, 得到完整的荧光信号, 用解卷积法反演植物荧光寿命.对活体法国冬青植株的光谱信息与荧光寿命特性进行了研究, 结果显示植物荧光寿命与叶绿素含量成线性关系, 并且植物叶绿素含量在0.03~0.06 mg/cm2这一特定范围, 线性相关系数高达0.9051, 比荧光光谱能更精确地反映植物叶绿素含量.
Abstract
Although the traditional fluorescence spectroscopy method can be used to evaluate the physiological status of plants, there are some measured defects, such as weak anti-jamming, large amount of data in plant remote sensing detection. Aiming at this matter, a fluorescence lifetime imaging technique is presented. The plant fluorescence lifetime and spectral information are combined to analyze the plant physiological information, and a wide spread of plant physiological status can be measured accurately. The many same fluorescence signal can be produced, while the plant is irradiated by continuous laser pulse in the time resolution measurement method. At the same time, a complete fluorescent signal will be got, while start-up delay time is changed constantly. Then, the plant fluorescence life can be retrieved by deconvolution arithmetic. The spectral information and fluorescence lifetime of living Viburnum awabuki plants was studied. Then, the results show that the plant fluorescence lifetime is linear with chlorophyll concentration. The linear correlation coefficient reaches into 0.9051 in specific range of 0.03~0.06 mg/cm2, and plant fluorescence lifetime is a better description of chlorophyll concentration than the fluorescence spectroscopy in the range.

万文博, 苏俊宏. 激光诱导植物叶绿素荧光寿命及光谱特性分析[J]. 光子学报, 2018, 47(6): 0630001. WAN Wen-bo, SU Jun-hong. Laser-induced Plant Chlorophyll Fluorescence Lifetime and Spectral Properties Analysis[J]. ACTA PHOTONICA SINICA, 2018, 47(6): 0630001.

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