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Fluorescence lifetime imaging of fluorescent proteins as an effective quantitative tool for noninvasive study of intracellular processes

Fluorescence lifetime imaging of fluorescent proteins as an effective quantitative tool for noninvasive study of intracellular processes

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Abstract

Fluorescence lifetime imaging (FLIM) is an effective noninvasive bioanalytical tool based on measuring fluorescent lifetime of fluorophores. A growing number of FLIM studies utilizes genetically engineered fluorescent proteins targeted to specific subcellular structures to probe local molecular environment, which opens new directions in cell science. This paper highlights the unconventional applications of FLIM for studies of molecular processes in diverse organelles of live cultured cells.

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DOI:10.1142/s1793545817300099

基金项目:this work were supported by the National Basic Research Program of China (2015CB352005); the National Natural Science Foundation of China (61525503/61378091/61620106016); Guangdong Natural Science Foundation Innovation Team (2014A030312008); Hong Kong, Macao and Taiwan cooperation innovation platform and major projects of international cooperation in Colleges and Universities in Guangdong Province (2015KGJHZ002); and Shenzhen Basic Research Project (JCYJ2015 0930104948169/JCYJ20160328144746940/GJHZ 20160226202139185).

收稿日期:2017-04-16

修改稿日期:2017-06-07

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Svitlana M. Levchenko:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province College of Optoelectronic Engineering, Shenzhen University Shenzhen, Guangdong Province 518060, P. R. China
Artem Pliss:Institute for Lasers, Photonics and Biophotonics University at Buffalo, State University of New York Buffalo, NY 14260-3000, USA
Junle Qu:Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province College of Optoelectronic Engineering, Shenzhen University Shenzhen, Guangdong Province 518060, P. R. China

联系人作者:Junle Qu(jlqu@szu.edu.cn)

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引用该论文

Svitlana M. Levchenko,Artem Pliss,Junle Qu. Fluorescence lifetime imaging of fluorescent proteins as an effective quantitative tool for noninvasive study of intracellular processes[J]. Journal of Innovative Optical Health Sciences, 2018, 11(1): 1730009

Svitlana M. Levchenko,Artem Pliss,Junle Qu. Fluorescence lifetime imaging of fluorescent proteins as an effective quantitative tool for noninvasive study of intracellular processes[J]. Journal of Innovative Optical Health Sciences, 2018, 11(1): 1730009

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