光学学报, 2018, 38 (10): 1006005, 网络出版: 2019-05-09
纳米膜修饰长周期光纤光栅生物传感特性研究 下载: 942次
Biosensing Properties of Nanofilm-Modified Long-Period Fiber Gratings
光纤光学 长周期光纤光栅 生物传感 纳米膜修饰光栅 低浓度测量 浓度灵敏度 fiber optics long-period fiber grating biosensing nanofilm-modified grating low-concentration measurement concentration sensitivity
摘要
针对长周期光纤光栅用作折射率传感器时对低浓度溶液不敏感的问题,提出了一种纳米膜修饰的光栅生物传感器,实现了抗原生物分子的低浓度测量。实验结果表明,经薄膜修饰后的光栅传感器,对低浓度的抗原溶液探测的灵敏度有了显著提高。当羊抗兔IgG浓度(质量浓度)为固定值0.01 mg·mL
-1时,随着兔IgG浓度的增加,覆膜长周期光纤光栅峰值损耗响应明显。实验测得周期为445,460,500 μm的生物传感器对应的浓度灵敏度分别为2101.5,1306.5,575.9 dB·mg
-1·mL。待测抗原浓度仅与光栅峰值损耗有关,实验测得兔IgG最小浓度为0.0003125 mg·mL
-1。该传感器灵敏度高、无需标记、稳定性强,且结构简单不易受电磁干扰,在生物传感领域拥有广阔的应用前景。
Abstract
We propose a nanofilm-modified grating biosensor that is able to detect low-concentration antigen biomolecules in order to find a solution for the insensitivity problem of the long-period fiber gratings (LPFGs) when used as refractive index sensors to detect low-concentration solutions. The experimental results show that the sensitivity of this biosensor is significantly improved. When the concentration of goat anti-rabbit IgG is fixed to 0.01 mg·mL
-1, the peak loss of the LPFG of the film indicates a significant response to the increase in rabbit IgG concentrations. Herein, the concentration is defined as a mass concentration. The biosensors successfully achieve concentration sensitivities of 2101.5, 1306.5, and 575.9 dB·mg
-1·mL measured at 445, 460, and 500 μm, respectively. Furthermore, the concentration of the antigen is related to the peak loss of the grating. The lowest detectable concentration of the rabbit IgG is 0.0003125 mg·mL
-1. Given the high sensitivity, free labeling, high stability, simple structure, and immunity from the electromagnetic interference, the sensor promises an excellent application prospect in the biosensing field.
齐跃峰, 贾翠, 刘磊, 张瑾, 刘燕燕, 王伟. 纳米膜修饰长周期光纤光栅生物传感特性研究[J]. 光学学报, 2018, 38(10): 1006005. Yuefeng Qi, Cui Jia, Lei Liu, Jin Zhang, Yanyan Liu, Wei Wang. Biosensing Properties of Nanofilm-Modified Long-Period Fiber Gratings[J]. Acta Optica Sinica, 2018, 38(10): 1006005.