本文采用偏振态显微成像系统对生物细胞热损伤进行监测。选取洋葱细胞、绿萝细胞作为实验样品, 对洋葱细胞、绿萝细胞进行热损伤, 通过比较细胞在热损伤前后的偏振态图像及其相应的偏振特性的变化, 分析热损伤细胞的偏振特性变化而获得相关信息。实验结果表明, 偏振态显微成像技术对于鉴别正常细胞和热损伤的细胞并评价细胞的损伤程度非常有效, 比显微光强成像更清晰地反映了细胞形态结构发生的变化, 在生物样品的特性研究及疾病诊断方面都有独特的优势, 为鉴别与评价细胞损伤程度提供一种快速的、无损的、有效的新方法。

The research designed a new method to evaluate thermal damaged cells. Polarization microscopic imaging was performed on onion cells and epipremnum aureum cells. By comparing and analyzing the changes in polarization characteristics of cells before and after thermal damage, relevant information is obtained. The experimental results indicate that the polarization microscopic imaging technique is effective in identifying normal cells and heat-damaged cells and evaluating the degree of thermal damage. Polarized microscopic imaging reflects changes in cell morphological structure more clearly than microscopic intensity imaging, it shows good development prospect in the study of biological samples and disease diagnosis. Polarization microscopic imaging technique is rapid, non-destructive, effective, and it is a new method for identifying and evaluating the degree of cell damage.