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Evaluation of growth characteristics of Aspergillus parasiticus inoculated in different culture media by shortwave infrared (SWIR) hyperspectral imaging

Evaluation of growth characteristics of Aspergillus parasiticus inoculated in different culture media by shortwave infrared (SWIR) hyperspectral imaging

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Abstract

The growth characteristics of Aspergillus parasiticus incubated on two culture media were examined using shortwave infrared (SWIR, 1000–2500 nm) hyperspectral imaging (HSI) in this work. HSI images of the A. parasiticus colonies growing on rose bengal medium (RBM) and maize agar medium (MAM) were recorded daily for 6 days. The growth phases of A. parasiticus were indicated through the pixel number and average spectra of colonies. On score plot of the first principal component (PC1 T and PC2, four growth zones with varying mycelium densities were identified. Eight characteristic wavelengths (1095, 1145, 1195, 1279, 1442, 1655, 1834 and 1929 nm) were selected from PC1 loading, average spectra of each colony as well as each growth zone. Furthermore, support vector machine (SVM) classifier based on the eight wavelengths was built, and the classification accuracies for the four zones (from outer to inner zones) on the colonies on RBM were 99.77%, 99.35%, 99.75% and 99.60% and 99.77%, 99.39%, 99.31% and 98.22% for colonies on MAM. In addition, a new score plot of PC2 and PC3 was used to differentiate the colonies incubated on RBM and MAM for 6 days. Then characteristic wavelengths of 1067, 1195, 1279, 1369, 1459, 1694, 1834 and 1929 nm were selected from the loading of PC2 and PC3. Based on them, a new SVM model was developed to differentiate colonies on RBM and MAM with accuracy of 100.00% and 99.99%, respectively. In conclusion, SWIR hyperspectral image is a powerful tool for evaluation of growth characteristics of A. parasiticus incubated in different culture media.

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DOI:10.1142/s1793545818500311

基金项目:This work was supported financially by the National Natural Science Foundation of China (No. 31772062) and Gannan Camellia Industry Development and Innovative Center Open Fund (Grant No. YK201610).

收稿日期:2018-03-15

修改稿日期:2018-08-13

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Xuan Chu:College of Engineering, China Agricultural University Beijing 100083, P. R. ChinaCollege of Mechanical and Electrical Engineering Zhongkai University of Agriculture Engineering Guangzhou 510225, P. R. China
Wei Wang:College of Engineering, China Agricultural University Beijing 100083, P. R. China
Xinzhi Ni:Crop Genetics and Breeding Research Unit USDA-ARS, 2747 Davis Road, Tifton, GA 31793, USA
Haitao Zheng:College of Food Science & Nutritional Engineering China Agricultural University, Beijing 100083, P, R. China
Xin Zhao:College of Engineering, China Agricultural University Beijing 100083, P. R. China
Hong Zhuang:Quality & Safety Assessment Research Unit U.S. National Poultry Research Center, USDA-ARS 950 College Station Rd., Athens, GA 30605, USA
Kurt C. Lawrence:Quality & Safety Assessment Research Unit USDA-ARS, Athens, GA 30605, USA
Chunyang Li:Institute of Food Science and Technology Jiangsu Academy of Agricultural Sciences Nanjing 210014, P. R. China
Yufeng Li:Multidisciplinary Initiative Center Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049, P. R. China
Chengjun Lu:Lingang Experimental Middle School Linyi 276624, P. R. China

联系人作者:Wei Wang(playerwxw@cau.edu.cn)

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引用该论文

Xuan Chu,Wei Wang,Xinzhi Ni,Haitao Zheng,Xin Zhao,Hong Zhuang,Kurt C. Lawrence,Chunyang Li,Yufeng Li,Chengjun Lu. Evaluation of growth characteristics of Aspergillus parasiticus inoculated in different culture media by shortwave infrared (SWIR) hyperspectral imaging[J]. Journal of Innovative Optical Health Sciences, 2018, 11(5): 1850031

Xuan Chu,Wei Wang,Xinzhi Ni,Haitao Zheng,Xin Zhao,Hong Zhuang,Kurt C. Lawrence,Chunyang Li,Yufeng Li,Chengjun Lu. Evaluation of growth characteristics of Aspergillus parasiticus inoculated in different culture media by shortwave infrared (SWIR) hyperspectral imaging[J]. Journal of Innovative Optical Health Sciences, 2018, 11(5): 1850031

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