激光与光电子学进展, 2018, 55 (12): 123001, 网络出版: 2019-08-01   

豆类种子自然老化过程的红外光谱研究 下载: 1162次

Infrared Spectroscopy of Naturally Aged Legume Seeds
作者单位
1 云南师范大学物理与电子信息学院, 云南 昆明 650500
2 曲靖师范学院物理与电子工程学院, 云南 曲靖 655011
摘要
老化种子的鉴定对农业生产和粮食安全具有重要意义。利用红外光谱三级鉴别法(傅里叶变换红外光谱技术、二阶导数红外光谱技术和二维相关红外光谱技术相结合)对不同储藏年份的豆类种子进行区分。结果显示:3种豆类种子的傅里叶变换红外光谱图总体相似,且主要由蛋白质和碳水化合物的吸收峰组成;不同储藏年份的豆类种子的原始红外光谱仅在1800~700 cm -1范围内有微小差异,几个吸收强度比随储藏年份增加呈现下降趋势;方差分析显示不同储藏年份的种子的吸光度比有显著差异;不同储藏年份的种子的二阶导数红外光谱(在1800~700 cm -1范围内)的吸收峰形状和强度存在差异;在二维相关红外光谱中,不同储藏年份的蚕豆和红豆种子(在860~1690 cm -1范围内)及大豆种子(在1350~1800 cm -1范围内)的自动峰和交叉峰出现的位置、数目及强度存在显著差异,其中数目随储藏年份增加而减少,强度随储藏年份增加而减弱。偏最小二乘法分析能对不同储藏年份的种子进行快速分类。3种豆类种子在老化过程中都表现出明显的光谱差异,表明红外光谱三级鉴别法可以快速区分不同储藏年份的豆类种子,有望发展成为简单、快速鉴别种子老化的光谱方法。
Abstract
The identification of seed aging has a great significance for agricultural production and food security. In this paper, three kinds of legume seeds with different storage years are distinguished by a tri-step IR spectra method, including Fourier transform infrared (FT-IR) spectroscopy, second derivative infrared (SD-IR) spectroscopy and two-dimensional correlation infrared (2D-IR) spectroscopy. The results show that the FT-IR spectra of three kinds of legume seeds are very similar, which are composed of the absorption peaks of protein and carbohydrate. But slight differences of original infrared spectrum of legume seeds with different storage years are observed in the range of 1800-700 cm -1. The absorption intensity ratios of three kinds of legume seeds show a decrease tendency with the increase of storage time. The variance analysis of the absorption intensity ratio show that the absorbance ratio of the seeds with different storage years is significantly different. More obvious differences are exhibited in their SD-IR spectra in the range of 1800-700 cm -1. In 2D-IR spectra, the positions, numbers and intensities of auto-peaks and cross-peaks are obvious different in the range of 860-1690 cm -1 for broad bean seeds and red kidney bean seeds, and in the range of 1350-1800 cm -1 for soybean seeds. The numbers decrease with the increase of storage years and the intensities weaken with the increase of storage years. Partial least squares analysis can accurately distinguish legume seeds with different storage years. Three kinds of legume seeds show obvious spectra differences, which demonstrate that FT-IR spectrum combined with SD-IR spectrum and 2D-IR spectrum can be used to discriminate legume seeds with different storage years. The method can provide a simple and rapid spectral method for detecting seed aging.

杨卫梅, 刘刚, 欧全宏, 安冉, 符致秋, 李建美, 时有明. 豆类种子自然老化过程的红外光谱研究[J]. 激光与光电子学进展, 2018, 55(12): 123001. Weimei Yang, Gang Liu, Quanhong Ou, Ran An, Zhiqiu Fu, Jianmei Li, Youming Shi. Infrared Spectroscopy of Naturally Aged Legume Seeds[J]. Laser & Optoelectronics Progress, 2018, 55(12): 123001.

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