Journal of Innovative Optical Health Sciences, 2009, 2 (1): 67–71, Published Online: Jan. 10, 2019  

OBSERVING NEURONAL ACTIVITIES WITH RANDOM ACCESS TWO-PHOTON MICROSCOPE

Author Affiliations
Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074, China
Abstract
As a second messenger in signal transduction, calcium ion plays a very important role in neuronal information processing and integrating. Limited by the imaging technique, it is difficult to simultaneously perform deep tissue imaging and measure intracellular free calcium concentration ([Ca2+]i) in different compartments of neurons in brain slice noncollinearly. By means of random access two-photon microscopy, which provides high optical penetration into tissues and low photo damage, we successfully measured [Ca2+]i of different parts of pyramidal neurons in neocortical layer V in rat brain slices with high spatial and temporal resolution. Combining the patch clamp technique, we stimulated the soma with depolarizing current and explored the dynamics of calcium in pyramidal neurons.

YUXIANG WU, XIULI LIU, WEI ZHOU, XIAOHUA LV, SHAOQUN ZENG. OBSERVING NEURONAL ACTIVITIES WITH RANDOM ACCESS TWO-PHOTON MICROSCOPE[J]. Journal of Innovative Optical Health Sciences, 2009, 2(1): 67–71.

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