Advanced Photonics, 2019, 1 (1): 016002, Published Online: Feb. 18, 2019   

Subvoxel light-sheet microscopy for high-resolution high-throughput volumetric imaging of large biomedical specimens Download: 1001次

Author Affiliations
1 Huazhong University of Science and Technology, School of Optical and Electronic Information, Wuhan, China
2 Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan, China
3 University of California, Los Angeles, Department of Bioengineering, Los Angeles, California, United States
4 University of Texas at Arlington, Joint Department of Bioengineering of UT Arlington/UT Southwestern, Arlington, Texas, United States
5 University of California, Los Angeles, School of Medicine, Los Angeles, California, United States
6 University of California, Los Angeles, Chemical and Biomolecular Engineering Department, Los Angeles, California, United States
7 Osaka Prefecture University, Nanoscience and Nanotechnology Research Center, Research Organization for the 21st Century, Osaka, Japan
8 University of California, Los Angeles, Mechanical and Aerospace Engineering Department, Los Angeles, California, United States
Abstract
A key challenge when imaging whole biomedical specimens is how to quickly obtain massive cellular information over a large field of view (FOV). We report a subvoxel light-sheet microscopy (SLSM) method enabling high-throughput volumetric imaging of mesoscale specimens at cellular resolution. A nonaxial, continuous scanning strategy is developed to rapidly acquire a stack of large-FOV images with three-dimensional (3-D) nanoscale shifts encoded. Then, by adopting a subvoxel-resolving procedure, the SLSM method models these low-resolution, cross-correlated images in the spatial domain and can iteratively recover a 3-D image with improved resolution throughout the sample. This technique can surpass the optical limit of a conventional light-sheet microscope by more than three times, with high acquisition speeds of gigavoxels per minute. By fast reconstruction of 3-D cultured cells, intact organs, and live embryos, SLSM method presents a convenient way to circumvent the trade-off between mapping large-scale tissue (>100 mm3) and observing single cell (∼1-μm resolution). It also eliminates the need of complicated mechanical stitching or modulated illumination, using a simple light-sheet setup and fast graphics processing unit-based computation to achieve high-throughput, high-resolution 3-D microscopy, which could be tailored for a wide range of biomedical applications in pathology, histology, neuroscience, etc.

Peng Fei, Jun Nie, Juhyun Lee, Yichen Ding, Shuoran Li, Hao Zhang, Masaya Hagiwara, Tingting Yu, Tatiana Segura, Chih-Ming Ho, Dan Zhu, Tzung K. Hsiai. Subvoxel light-sheet microscopy for high-resolution high-throughput volumetric imaging of large biomedical specimens[J]. Advanced Photonics, 2019, 1(1): 016002.

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