光学学报, 2019, 39 (2): 0211004, 网络出版: 2019-05-10
谱域相位显微成像的相位解包裹 下载: 1020次
Phase Unwrapping in Spectral Domain Phase Microscopy
成像系统 谱域相位显微成像 定量相位成像 相位解包裹 合成波长 imaging systems spectral domain phase microscopy quantitative phase imaging phase unwrapping synthetic wavelength
摘要
提出了一种应用于谱域相位显微成像的相位解包裹方法。利用傅里叶变换及合成波长相位计算方法分别得到具有较小噪声的包裹相位和具有较大噪声的解包裹相位,利用解包裹相位与包裹相位之差计算包裹相位的包裹次数,以此对具有较小噪声的包裹相位进行解包裹。该方法消除了现有方法引入的边界分段错误。建立了一种基于合成波长的谱域相位显微成像系统,使用压电位移台定量验证了该系统可以用于大梯度边界的相位解包裹,并进行了红细胞和倾斜镜面的相位成像。该系统在空气中的位移灵敏度为0.043 nm。
Abstract
A phase unwrapping method used in the spectral domain phase microscopy (SDPM) is proposed. A wrapped phase with a small noise and an unwrapped phase with a big noise are obtained by using respectively the Fourier transform (FT) method and the synthetic-wavelength phase calculation method. The wrapped number of the wrapped phase is calculated from the difference between the unwrapped and wrapped phases, and thus the unwrapping of the wrapped phase with a small noise is conducted. The presented method eliminates the boundary segmentation error introduced in the existing phase unwrapping methods. An SDPM system based on synthetic-wavelength is established. A piezoelectric translation stage is used to quantitatively verify that this system is capable of accurately unwrapping the phase with a large gradient boundary. The phase imaging of red blood cells and tilted mirror surfaces is also performed. The displacement sensitivity of this system in air is 0.043 nm.
周红仙, 朱礼达, 赵玉倩, 马振鹤, 王毅. 谱域相位显微成像的相位解包裹[J]. 光学学报, 2019, 39(2): 0211004. Hongxian Zhou, Lida Zhu, Yuqian Zhao, Zhenhe Ma, Yi Wang. Phase Unwrapping in Spectral Domain Phase Microscopy[J]. Acta Optica Sinica, 2019, 39(2): 0211004.