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基于量子点荧光猝灭法的蛋白质检测芯片

Protein Detection Chip Based on Quantum Dot Fluorescence Quenching Method

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摘要

基于羧基量子点的荧光猝灭效应,提出了一种检测神经生长因子(NGF)的新方法。研究结果表明,在pH7.5的缓冲溶液中,NGF抗原与羧基量子点标记的NGF抗体发生特异性结合,能使羧基量子点的荧光发生猝灭。在1~20 ng·mL-1范围内,荧光猝灭程度与NGF抗原质量浓度呈良好的线性关系,线性系数为0.9696,检测下限为1 ng·mL-1。所提检测方法具有良好的抗干扰特性,在诊断检测方面具有巨大的应用潜力。

Abstract

A new method for detecting the nerve growth factors (NGF) is proposed based on the fluorescence quenching effect of carboxyl quantum dots. The research results show that in the pH7.5 buffer solution, the NGF antigen specifically binds to the carboxyl quantum dot-labeled NGF antibody, which can quench the fluorescence of the carboxyl quantum dots. The fluorescence quenching degree and the NGF antigen mass concentration has a good linear relationship in the range of 1-20 ng/mL, and the linear correlation coefficient is 0.9696 with the detection limit of 1 ng/mL. This proposed method has good anti-interference characteristics and has great potential applications in diagnosis and detection.

Newport宣传-MKS新实验室计划
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中图分类号:O432

DOI:10.3788/lop56.062601

所属栏目:物理光学

基金项目:国家自然科学基金(11204046)、国家国际科技合作专项项目(2014DFA00670)、中央引导地方科技发展专项(QKZYD[2017]4004)、贵州省科技支撑项目(QKHZ[2017]2887)

收稿日期:2018-08-15

修改稿日期:2018-09-05

网络出版日期:2018-09-30

作者单位    点击查看

彭嫚:贵州大学大数据与信息工程学院, 贵州 贵阳 550025贵州师范大学机械与电气工程学院, 贵州 贵阳 550025
白忠臣:贵州大学贵州省光电子技术及应用重点实验室,贵州 贵阳 550025
张莹:贵州大学贵州省光电子技术及应用重点实验室,贵州 贵阳 550025
黎显继:贵州大学贵州省光电子技术及应用重点实验室,贵州 贵阳 550025
张正平:贵州大学贵州省光电子技术及应用重点实验室,贵州 贵阳 550025

联系人作者:张正平(pengman1001@163.com)

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引用该论文

Peng Man,Bai Zhongchen,Zhang Ying,Li Xianji,Zhang Zhengping. Protein Detection Chip Based on Quantum Dot Fluorescence Quenching Method[J]. Laser & Optoelectronics Progress, 2019, 56(6): 062601

彭嫚,白忠臣,张莹,黎显继,张正平. 基于量子点荧光猝灭法的蛋白质检测芯片[J]. 激光与光电子学进展, 2019, 56(6): 062601

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