微生物检测在医疗诊断、食品安全、发酵工程、微生物科学研究等方面具有重要意义。 微生物检测主要体现两个方面：微生物的判断和微生物生长曲线的测量。微生物的生长通常伴随着一系列与生长量相平行的DNA、酸 碱度等生理指标和CO2、光学厚度等产物指标,这些指标为微生物检测提供了测量途径。其中,通过检测微生物代谢产物CO2, 不仅可以排除死菌带来的干扰,而且能够实现快速、全自动检测,成为微生物检测的主要途径之一。可调谐半导体二极管激光吸收光谱技术(TDLAS)以其在测量CO2的灵敏度高、 结构简单、技术成熟等优势,为微生物检测提供一种快速、非侵入的新检测途径,对推动微生物检测技术的发展具有重要的意义。 介绍了近几年TDLAS技术及其在微生物(生长)检测领域应用取得的进展。

Microbial monitoring plays a key role in a wide range of fields spanning from medical diagnosis and food safety to fermentation and microbiological research. Microbial monitoring mainly include two aspects: the judgment of microorganisms and the measurement of the growth curve of microorganisms. Microbial growth usually correlate directly with certain indicators, such as DNA, pH, CO2 and OD, which provide a way for microbial monitoring. Among them, CO2 measurements have become the preferred approach because they prevent the interference of the dead bacteria and provide a more rapid and automatic answer. TDLAS technology has demonstrated high sensitivity, while being a simple yet mature technological structure for the detection of CO2 to provide a rapid and non-intrusive technique for microbial monitoring. It is of great significance to promote a new approach for monitor microorganisms. Progress of TDLAS technology in the field of microbial monitoring(growth) in recent years are reviewed.