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基于图像拼接的高通量数字PCR荧光基因芯片读取系统的设计

Design of high throughput digital PCR fluorescence gene chip detection system based on image mosaic

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摘要

针对高通量数字聚合酶链式反应荧光基因芯片检测的需求,提出了一种基于荧光显微光学技术的基因芯片检测系统。系统以无限远荧光显微系统为框架,通过制冷CCD一次完成较大视场的成像,顺序移动基因芯片得到全部图像,通过图像拼接完成检测,切换二向色镜组实现检测不同荧光通道的目的。光学系统分辨率可达16.3μm、曝光时间500ms,目前只需要拼接35次,即可在1min内完成对28mm×16mm的基因芯片内两万多荧光通道的检测,极大的提高了检测效率。

Abstract

In face of the requirement of the detection of high throughput digital polymerase chain reaction (dPCR) fluorescence gene chip, a new optical detection system based on fluorescence microscopy is proposed. The design of this system is depending on an afocal fluorescence microscopy system. It can complete the imaging of the larger field of view through refrigerating CCD. All the images can be got by moving the gene chip in sequence, and the complete image can be obtained through image splicing. By changing the dichroic mirror and filter, the detection system can detect different kinds of fluorescence gene chip. The optical system has a resolution of 20mm, an exposure time of 500ms and 35 times of splicing. It can complete the detection of the gene chip with more than 20000 channel within 1 minutes, and the area of the gene chip is 28mm×18mm. This system greatly reduces the detection time.

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中图分类号:O439

所属栏目:生物光学

基金项目:上海市科学技术委员会地方院校能力建设项目-高通量PCR基因芯片荧光检测系统研制(18060502500)

收稿日期:2018-05-16

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作者单位    点击查看

朱天赟:上海理工大学 光电信息与计算机学院, 上海  200093上海理工大学 上海市现代光学系统重点实验室, 上海  200093
郑继红:上海理工大学 光电信息与计算机学院, 上海  200093上海理工大学 上海市现代光学系统重点实验室, 上海  200093
孙刘杰:上海理工大学 上海市现代光学系统重点实验室, 上海  200093上海理工大学 出版印刷与艺术设计学院, 上海  200093
万新军:上海理工大学 光电信息与计算机学院, 上海  200093上海理工大学 上海市现代光学系统重点实验室, 上海  200093
黄新荣:上海理工大学 光电信息与计算机学院, 上海  200093上海理工大学 上海市现代光学系统重点实验室, 上海  200093

联系人作者:朱天赟(15121144283@163.com)

备注:朱天赟(1994-),男 ,硕士研究生,从事光学系统设计研究。

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引用该论文

ZHU Tianyun,ZHENG Jihong,SUN Liujie,WAN Xinjun,HUANG Xinrong. Design of high throughput digital PCR fluorescence gene chip detection system based on image mosaic[J]. Optical Technique, 2019, 45(1): 107-111

朱天赟,郑继红,孙刘杰,万新军,黄新荣. 基于图像拼接的高通量数字PCR荧光基因芯片读取系统的设计[J]. 光学技术, 2019, 45(1): 107-111

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