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荧光分析法在血糖检测中的应用

Application of fluorescence spectrum analysis in blood glucose detection

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摘要

用不同波长的光激发葡萄糖溶液和血清样品,确定血清的最佳激发波长。以相同波长的光激发含不同血糖浓度的血清样品,得到不同血清的荧光发射光谱,通过分析其荧光光谱判断血清中的血糖浓度大小。实验结果表明,检测血清的最佳激发波长为340nm,血清中葡萄糖的发射峰位置为470nm; 随着血清中葡萄糖浓度的增加,荧光光谱图中荧光峰处荧光强度总体呈上升趋势,光谱的面积积分强度和导数光谱中一阶导数的最大值也呈上升趋势,荧光峰的半峰宽整体呈现下降趋势。荧光光谱分析法操作简单,灵敏度高,费用相对较低,为血糖检测提供了有力的参考。

Abstract

With the glucose solution and serum excited by light in different wavelength, the optimum excitation wavelength of serum was determined. After exciting the serum containing different blood glucose levels with the same light wavelength, this paper obtained the fluorescence emission spectrum of different serum,and then estimated the blood glucose level in the method of analyzing its fluorescence spectrum. The experimental results have demonstrated that the optimal excitation wavelength detecting serum is 340nm and the emission peak of glucose in serum appears at 470nm. With the increase of glucose levels, the fluorescence intensity in the fluorescence peak shows an upward trend in the fluorescence spectrum. Besides, there is also growth tendency in the area integrals and the maximum value of the first-order derivative in the derivative spectrum, while the full width at half maximum of the fluorescence peak shows a downward trend as a whole. With the advantages of simple operation, high sensitivity and low cost, fluorescence spectrum analysis provides a method for the detection of blood glucose.

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中图分类号:O433.4;O657.3

所属栏目:生物光学

基金项目:国家自然科学基金(61308115);上海市自然科学基金(13ZR1457900):上海市科委产学研医项目(15DZ1940400)

收稿日期:2017-11-09

修改稿日期:2018-01-08

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陶建峰:上海理工大学 教育部现代微创医疗器械及技术工程研究中心 生物医学光学与视光学研究所, 上海  200093
陈明惠:上海理工大学 教育部现代微创医疗器械及技术工程研究中心 生物医学光学与视光学研究所, 上海  200093
郑刚:上海理工大学 教育部现代微创医疗器械及技术工程研究中心 生物医学光学与视光学研究所, 上海  200093
王成:上海理工大学 教育部现代微创医疗器械及技术工程研究中心 生物医学光学与视光学研究所, 上海  200093
宋云霄:上海市徐汇中心医院, 上海  200031

联系人作者:陶建峰(taojianfeng1314@sina.com)

备注:陶建峰(1990-),男,硕士研究生,从事生物医学光子学研究。

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引用该论文

TAO Jianfeng,CHEN Minghui,ZHENG Gang,WANG Cheng,SONG Yunxiao. Application of fluorescence spectrum analysis in blood glucose detection[J]. Optical Technique, 2019, 45(1): 124-128

陶建峰,陈明惠,郑刚,王成,宋云霄. 荧光分析法在血糖检测中的应用[J]. 光学技术, 2019, 45(1): 124-128

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