首页 > 论文 > Journal of Innovative Optical Health Sciences > 12卷 > 2期(pp:1950004--1)

Optimization method to suppress background for imaging multiple planes

Optimization method to suppress background for imaging multiple planes

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

Abstract

3D live imaging is important for better understanding of biological processes. To obtain biological dynamic process, high imaging speed is required. In order to improve speed in 3D live imaging, simultaneous imaging of multiple planes throughout a 3D volume has been proposed. However, a main disadvantage of this method is the cross-talk from neighboring imaging planes. In this paper, we propose an optimization method to suppress background from neighboring imaging planes. A D-aperture is used to generate multiple light sheets. An optimization method to suppress background is presented. The simulation results demonstrated that the proposed method can be used to suppress the effectiveness of background from neighboring light sheets.

Newport宣传-MKS新实验室计划
补充资料

DOI:10.1142/s1793545819500044

基金项目:This work is supported by the Vietnam National Foundation for Science and Technology Development (NAFOSTED) underGrant number (103.03-2018.08).

收稿日期:2018-03-19

修改稿日期:2018-12-12

网络出版日期:--

作者单位    点击查看

Vannhu Le:Department of Optical Engineering, Le Quy Don Technical University, 236 Hoang Quoc Viet Street, Tu Liem District, Hanoi, VietnamState Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, P. R. ChinaInstitute of Research and Development, Duy Tan University, Da Nang 550000, Vietnam

联系人作者:Vannhu Le(levannhu_mta2015@yahoo.com)

【1】P. Gao, B. Prunsche, L. Zhou, K. Nienhaus, G. U. Nienhaus, “Background suppression in fluorescence nanoscopy with stimulated emission double depletion,” Nat. Photonic. 11, 163–169 (2017).

【2】B. Huang, W. Wang, M. Bates, X. Zhuang, “Three-dimensional super-resolution imaging by stochastic optical reconstruction microscopy,” Science 319, 810–813 (2008).

【3】B. Richards, E. Wolf, “Electromagnetic diffraction in optical systems. Structure of the image field in an aplanatic system,” Proc. R. Soc. A 253, 358–379 (1959).

【4】R. Loic, C. L. William, K. C. Raghav, W. Yinan, C. Michael, W. M. Eugene, J. K. Philipp, “Adaptive light sheet microscopy for long-term, high resolution imaging in living organisms,” Nat. Biotechnol. 12, 1267–1281 (2016).

【5】E. Fuchs, J. S. Jaffe, “Thin laser light sheet microscopy for microbial oceanography,” Opt. Exp. 10(2), 145 (2002).

【6】M. Friedrich, Q. Gan, V. Ermolayev, G. S. Harms, “STED-SPIM: Stimulated emission depletion improves sheet illumination microscopy resolution,” Biophys. J. 100(8), L43–L45 (2011).

【7】T. F. Holekamp, D. Turaga, T. E. Holy, “Fast three-dimensional fluorescence imaging of activity in neural populations by objective coupled planar illumination microscopy,” Neuron. 57(5), 661–672 (2008).

【8】L. Silvestri, A. Bria, L. Sacconi, G. Lannello, F. S. Pavone, “Confocal light sheet microscopy: Micron-scale neuroanatomy of entire mouse brain,” Opt. Exp. 18, 20482–20598 (2012).

【9】R. Itoh, J. R. Landry, S. Hamann, O. Solgaard, “Light sheet fluorescence microscopy using high-speed structured and pivoting illumination,” Opt. Lett. 41(21), 5015–5018 (2016).

【10】K. Mohan, S. B. Purnapatra, P. P. MOndal, “Three dimensional fluorescence imaging using multiple light sheet microscopy,” Plos 39, 4715 (2014).

【11】A. K. Gustavsson, P. N. Petrov, M. Y. Lee, Y. Shechtman, W. E. Moerner, “3D single-molecule super-resolution miecroscopy with a tilted light sheet,” Nature Communications 9, 123 (2018).

【12】L. Gao, L. Shao, B. C. Chen, E. Betzig, “3D live fluorescence imaging of cellular dynamic using Bessel beam plane illumination microscopy,” Protocol. 9(5), 1083 (2014).

【13】B. C. Chen, W. R. Legant, K. Wang, L. Shao, D. E. Milkie, M. W. Davidson, C. Janetopoulos, X. S. Wu, J. A. Hammer III, Z. Liu, B. P. English, Y. Mimori-Kiyosue, D. P. Romero, A. T. Riter, J. Lippincott-Schwartz, L. Firtz-Laylin, R. D. Mullins, D. M. Mitchell, J. N. Bembenek, A. C. Reymann, R. Bohme, S. W. Grill, J. T. Wang, G. Seydoux, U. S. Tulu, D. P. Kiehart, E. Betzig, “Lattice light-sheet microscopy: Imaging molecules to embryos at high spatiotemporal resolution,” Science 346, 1257998 (2014).

【14】T. Vettenburg, H. I. C. Dalgarno, J. Nylk, C. Coll-Llado, D. E. K. Ferrier, T. Czmar, F. J. Gunn-Moore, K. Dholakia, “Light sheet microscopy using an Airy beam,” Nat. Methods 11, 541–544 (2014).

【15】M. Duocastella, C. B. Arnold, J. Puchalla, “Selectable light-sheet uniformity using tuned a xial scanning,” Microsc. Res. Tech. 80, 250–259 (2017).

【16】U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, L. Hufnagel, “Multiview light sheet microscopy for rapid in toto imaging,” Nat. Meth. 9(7), 730 (2012).

【17】R. Tomer, M. Lovett-Barron, I. Kauvar, A. Andalman, V. M. Burns, S. Sankaran, L. Grosenick, M. Broxton, S. Yang, K. Deisseroth, “SPED light sheet microscopy: Fast mapping of biological system structure and function,” Cell 163, 1796 (2015).

【18】O. E. Olarte, J. Andlla, D. Artigas, P. Loza-Alvares, “Decoupled illumination detection in light sheet microscopy for fast volumetric imaging,” Optica 2(8), 702 (2015).

【19】F. O. Fahrbach, F. F. Voigt, B. Schmid, F. Helmchen, J. Huisken, “Rapid 3D light-sheet microscopy with a tunable lens,” Opt. Exp. 21, 21010–21026 (2013).

【20】S. Kumar, D. Wilding, M. B. Sikkel, A. R. Lyon, K. T. Macleod, C. Dunsby, “High-speed 2D and 3D fluorescence microscopy of cardiac myocytes,” Opt. Exp. 19, 13839 (2011).

【21】M. B. Bouchard, V. Voleti, C. S. Mendes, C. Lacefield, W. B. Grueber, R. S. Mann, R. M. Bruno, E. M. C. Hillman, “Swept confocally-aligned planar excitation (SCAPE) microscopy for high-speed volumetric imaging of behaving organisms,” Nat. Photonics 9, 113 (2015).

【22】E. J. Bothcherby, C. W. Smith, M. M. Kohl, D. Debarre, M. J. Booth, R. Juskaitis, O. Paulsen, T. Wilson “Aberration-free three-dimensional multiphoton imaging of neuronal activity at kHz rates,” Proc. Natl. Acad. Sci. USA 109, 2919 (2012).

【23】K. M. Dean, P. Roudot, E. S. Welf, T. Pohlkamp, G. Garrelts, J. Herz, R. Folka, “Imaging subcellular dynamics with fast and light-efficient volumetrically parallelized microscopy,” Optica 4(2), 263 (2017).

【24】P. P. Mondal, S. Dilipkumar, K. Mohan, “Efficient generation of diffraction-limited multi-sheet pattern for biological imaging,” Opt. Lett. 40(4), 609 (2015).

【25】S. Geissbuehler, A. Sharipov, A. Godinat, N. L. Bocchio, P. A. Sandoz, A. Huss, N. A. Jensen, S. Jakobs, J. Enderlein, F. Gisou van der Goot, E. A. Dubikovskaya, T. Lasser, M. Leutenegger, “Live-cell mutiplane three-dimensional super-resolution optical fluctuation imaging,” Nat. Commun. 5, 5830 (2014).

【26】Q. Ma, B. Khademhosseinieh, E. Huang, H. Qian, M. A. Bakowski, E. R. Troemel, Z. Liu, “Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes,” Sci. Rep. 6, 31445 (2016).

【27】B. Hajj, J. Wisniewski, M. EI Beheiry, J. Chen, A. Revyakin, C. Wu, M. Dahan, “Whole-cell, muiticolor superresolution imaging using mutifocus microscopy,” Proc. Natl. Acad. Sci. USA 11, 17480 (2014).

引用该论文

Vannhu Le. Optimization method to suppress background for imaging multiple planes[J]. Journal of Innovative Optical Health Sciences, 2019, 12(2): 1950004

Vannhu Le. Optimization method to suppress background for imaging multiple planes[J]. Journal of Innovative Optical Health Sciences, 2019, 12(2): 1950004

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF