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Combined spatial frequency spectroscopy analysis with visible resonance Raman for optical biopsy of human brain metastases of lung cancers

Combined spatial frequency spectroscopy analysis with visible resonance Raman for optical biopsy of human brain metastases of lung cancers

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Abstract

The purpose of this study is to examine optical spatial frequency spectroscopy analysis (SFSA) combined with visible resonance Raman (VRR) spectroscopic method, for the first time, to discriminate human brain metastases of lung cancers adenocarcinoma (ADC) and squamous cell carcinoma (SCC) from normal tissues. A total of 31 label-free micrographic images of three types of brain tissues were obtained using a confocal micro-Raman spectroscopic system. VRR spectra of the corresponding samples were synchronously collected using excitation wavelength of 532nm from the same sites of the tissues. Using SFSA method, the difference in the randomness of spatial frequency structures in the micrograph images was analyzed using Gaussian function fitting. The standard deviations, σ calculated from the spatial frequencies of the micrograph images were then analyzed using support vector machine (SVM) classifier. The key VRR biomolecular fingerprints of carotenoids, tryptophan, amide II, lipids and proteins (methylene/methyl groups) were also analyzed using SVM classifier. All three types of brain tissues were identified with high accuracy in the two approaches with high correlation. The results show that SFSA–VRR can potentially be a dual-modal method to provide new criteria for identifying the three types of human brain tissues, which are on-site, real-time and label-free and may improve the accuracy of brain biopsy.

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DOI:10.1142/s179354581950010x

基金项目:This research is supported by The Air Force Medical Center, China and in part of The Institute for Ultrafast Spectroscopy and Lasers (IUSL), the City College of the City University of New York.

收稿日期:2018-12-16

修改稿日期:2019-03-04

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Yan Zhou:The Air Force Medical Center, PLA, No. 30, Fuchenglu, Haidian District, Beijing 100142, P. R. China
Cheng-Hui Liu:Department of Physics, The City College of the City, University of New York, Institute of Ultrafast Spectroscopy and Lasers, NY 10031, USA
Yang Pu:Department of Physics, The City College of the City, University of New York, Institute of Ultrafast Spectroscopy and Lasers, NY 10031, USA
Binlin Wu:Physics Department and CSCU Center for Nanotechnology, Southern Connecticut State University, 501 Crescent Street, New Haven, CT 06515, USA
Thien An Nguyen:Department of Physics, The City College of the City, University of New York, Institute of Ultrafast Spectroscopy and Lasers, NY 10031, USA
Gangge Cheng:The Air Force Medical Center, PLA, No. 30, Fuchenglu, Haidian District, Beijing 100142, P. R. China
Lixin Zhou:Beijing Cancer Hospital, No. 52, Fuchenglu, Haidian District, Beijing 100142, P. R. China
Ke Zhu:Institute of Physics, Chinese Academy of Sciences (CAS), Beijing 100190, P. R. China
Jun Chen:Department of Cardiology, Tianjin Medical, University General Hospital, Tianjin 300052, P. R. China
Qingbo Li:Beihang University, Xueyuan Road, No. 37, Haidian District, Beijing 100191, P. R. China
Robert R. Alfano:Department of Physics, The City College of the City, University of New York, Institute of Ultrafast Spectroscopy and Lasers, NY 10031, USA

联系人作者:Robert R. Alfano(ralfano@ccny.cuny.edu)

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引用该论文

Yan Zhou,Cheng-Hui Liu,Yang Pu,Binlin Wu,Thien An Nguyen,Gangge Cheng,Lixin Zhou,Ke Zhu,Jun Chen,Qingbo Li,Robert R. Alfano. Combined spatial frequency spectroscopy analysis with visible resonance Raman for optical biopsy of human brain metastases of lung cancers[J]. Journal of Innovative Optical Health Sciences, 2019, 12(2): 1950010

Yan Zhou,Cheng-Hui Liu,Yang Pu,Binlin Wu,Thien An Nguyen,Gangge Cheng,Lixin Zhou,Ke Zhu,Jun Chen,Qingbo Li,Robert R. Alfano. Combined spatial frequency spectroscopy analysis with visible resonance Raman for optical biopsy of human brain metastases of lung cancers[J]. Journal of Innovative Optical Health Sciences, 2019, 12(2): 1950010

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