针对出现的三等位基因2例亲子鉴定案件, 同时检测其唾液斑及毛发, 应用Goldeneye 20A、 PowerPlex 6C、Investigator 24plex QS及华夏白金试剂盒进行检测验证。结果显示在2例二联体亲子鉴定案件中, 一例母亲的D18S51基因座分型为13/16, 孩子的分型为15/16/17; 另一例父亲的D7S820基因座分型为8/11/12, 孩子的分型为10/11。唾液斑及毛发样本结果与FTA血卡结果一致, 应用Goldeneye 20A、PowerPlex 6C 系统、Investigator 24plex QS及华夏白金试剂盒验证检测的结果相同。在日常亲子鉴定案件中, 出现三带型现象较为罕见, 针对这种现象, 需进行认真判读及确认, 以保证检测结果的准确性。

Two cases of three-banded pattern were detected with their saliva spots and hairs by Goldeneye 20A kit, PowerPlex 6C, Investigator 24plex QS and Huaxia Platinum kit. One case of tri-allele in children in D18S51(15/16/17) and the type of this loci of the children’s mother is 13 and 16, another case of tri-allele in father in D7S820(8/11/12) and the child is 10 and 11 were genotyped with Goldeneye 20A kit. The results of saliva spot and hair samples are consistent with those of FTA blood card.The results were the same as those by PowerPlex 6C, Investigator 24plex QS and Huaxia Platinum kit. More attention should be paid to the paternity testing because of the rare three-banded patterns of STR loci.