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Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues

Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues

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Abstract

The endogenous fluorophores such as reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD) in cells and tissues can be imaged by fluorescence lifetime imaging microscopy (FLIM) to show the tissue morphology features, as well as the biomolecular changes in microenvironment. The two important coenzymes in cellular metabolism, NAD(P)H and FAD, can be used to monitor the cellular metabolic status. This work proposed a novel method to study the uterine metabolism at the adjacent site of healthy cervix. It was found that the benign uterine tumors such as leiomyomas and adenomyosis with abnormal cell growth can be detected by measuring the fluorescence lifetime of NAD(P)H and FAD in adjacent healthy cervical tissues. This method opened a novel strategy for afflicted women to undergo the cervical biopsies instead of hysterectomies for detecting tumors, which can preserve the fertility of patients. The FLIM studying on NAD(P)H and FAD indicated the correlation between metabolism and some diseases, including diabetes, hyperthyroidism and obesity. It was also suggested that the metabolic level might be quite different for a patient with a malignant tumor history.

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DOI:10.1142/s1793545819400066

基金项目:This work is supported by the National Natural Science Foundation of China (NSFC) (Grant Nos. 11574056, 61575046 and 11804350), Ministry of science and technology of the People's Republic of China (China-Serbia bilateral project SINO-SERBIA2018002), the Shanghai Sailing Program (Grant No. 17YF1421300), Fudan University-CIOMP Joint Fund (FC2017-007, FC2018-001), and Pioneering Project of Academy for Engineering and Technology, Fudan University (gyy2018-001, gyy2018-002). We also thank all the patients involved in this study.

收稿日期:2019-04-29

修改稿日期:2019-07-30

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Maojia Huang:Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. China
Zixiao Zhang:Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. China
Xinyi Wang:Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. China
Yonghui Xie:Department of Pathology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430014, P. R. China
Yiyan Fei:Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. China
Jiong Ma:Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. China
Jing Wang:State Key Laboratory of High Field Laser Physics, Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, P. R. China
Li Chen:School of Arts and Sciences, MCPHS University, Boston, MA 02115, U. S. A.
Lan Mi:Department of Optical Science and Engineering, Shanghai Engineering Research Center of Ultra-precision Optical Manufacturing, Fudan University, Shanghai 200433, P. R. China
Yulan Wang:Department of Gynecology, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology, Wuhan 430014, P. R. China

联系人作者:Yulan Wang(wyl407@163.com)

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引用该论文

Maojia Huang,Zixiao Zhang,Xinyi Wang,Yonghui Xie,Yiyan Fei,Jiong Ma,Jing Wang,Li Chen,Lan Mi,Yulan Wang. Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1940006

Maojia Huang,Zixiao Zhang,Xinyi Wang,Yonghui Xie,Yiyan Fei,Jiong Ma,Jing Wang,Li Chen,Lan Mi,Yulan Wang. Detecting benign uterine tumors by autofluorescence lifetime imaging microscopy through adjacent healthy cervical tissues[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1940006

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