随着工农业的发展, 各类材料如纳米材料、重金属材料应用, 及其他一些生物质类材料的积累, 对生物体的生长发育和繁殖等都有着较明显的生理毒理效应。只有对这些材料的生物效应有了清楚的了解才能正确地使用而避免它对人体产生毒副作用。本试验以秀丽隐杆线虫为研究对象, 从生长发育到基因水平, 考察了CdS/ZnS量子点对的生物安全性。研究发现CdS/ZnS量子点暴露不仅对秀丽隐杆线虫的体长、头部摆动以及身体弯曲等生物行为有不同程度的抑制作用, 而且对线虫细胞毒性相关基因、胁迫相关基因以及细胞生长发育相关基因的表达也有一定的影响。当CdS/ZnS量子点的暴露浓度为3.33 nmol/L, 线虫体长、头部摆动以及身体弯曲分别从空白组的(0.64±0.072)mm, (55.94±7.17)次/min和(9.95±2.42)次/20 s降到(0.48±0.099)mm, (15.83±6.76)次/min和(7.72±1.12)次/20 s; 而GST-1, daf-21, HSP-16.4等基因表达分别增加了15.33, 19.51 和 35.01倍。本研究为量子点在应用过程中的生物安全性研究提供理论依据。

With the rapid development of industrialization and urbanization, more and more materials including nanomaterials, heavy metals and biomass materials have been exposed in our life. In order to study the influencing mechanism of these materials in biological tissue, we investigated the biosafety of quantum dots materials. The aims were to assess physiological behavior and some gene expressions of Caenorhabditis elegans with quantum dots CdS/ZnS expoure. The results showed that the quantum dots inhited the biological behavior of Caenorhabditis elegans, and up-regulated the expression of some genes including the cytotoxic gene, stress-related gene and development related gene.The body length, head-swimming rate and body bending of C.elegans was down to (0.48±0.099) mm, (15.83±6.76) times/min and (7.72±1.12) times/20 s, from the control of (0.64±0.072) mm,(55.94±7.17) times/min and (9.95±2.42) times/20 s respectviely. And, the gene transcript of GST-1, daf-21, HSP-16.4 was up-regulation to 15.33, 19.51 and 35.01 folds respectviely with 3.33 nmol/L CdS/ZnS quantum dots exposure. This work highlights the utility of the Caenorhabditis elegans model as a multiflexible platform to allow noninvasively imaging and monitoring in vivo consequences of engineered nanomaterials.