Photonic Sensors, 2019, 9 (4): 309, Published Online: Dec. 5, 2019
A High-Sensitivity and Broad-Range SPR Glucose Sensor Based on Improved Glucose Sensitive Membranes
Surface plasmon resonance sensor glucose sensitive membrane immobilized enzyme silica mesocellular foams
Abstract
An improved glucose sensitive membrane (GSM) is prepared by immobilizing glucose oxidase (GOD) onto a mixture of silica mesocellular foams (SiMCFs) and SiO2 nanoparticles (SiNPs) and then trapping it in a polyvinyl alcohol (PVA) gel. The membrane is coated onto a gold-glass sheet to create a surface plasmon resonance (SPR) sensor. A series of experiments are conducted to determine the optimized parameters of the proposed GSM. For a GSM with a component ratio of SiMCFs : SiNPs = 7 : 3 (mass rate), the resonance angle of the sensor decreases from 68.57° to 63.36°, and the average sensitivity is 0.026°/(mg/dL) in a glucose concentration range of 0 mg/dL-200 mg/dL. For a GSM with a component ratio of SiMCFs : SiNPs = 5 : 5 (mass rate), the resonance angle of the sensor decreases from 67.93° to 63.50°, and the sensitivity is 0.028°/(mg/dL) in a glucose concentration range of 0 mg/dL - 160 mg/dL. These data suggest that the sensor proposed in this study is more sensitive and has a broader measurement range compared with those reported in the literature to date.
Yinquan YUAN, Na YUAN, Dejing GONG, Minghong YANG. A High-Sensitivity and Broad-Range SPR Glucose Sensor Based on Improved Glucose Sensitive Membranes[J]. Photonic Sensors, 2019, 9(4): 309.