为实现细菌的快速、低成本、高通量检测, 设计并制备了一种阵列式柔性纸基SERS检测芯片。首先制备银溶胶, 再利用激光打印和碳粉的疏水性, 在纸基上分别构造隔离区与阵列检测区。在检测区循环滴加银溶胶, 由于碳粉的疏水特性, 将银溶胶液滴限制在检测区范围。一定温度下, 银溶胶自然干燥即形成阵列式活性SERS检测芯片。利用罗丹明6G作为探针分子对纸基SERS芯片进行了表征, 测试结果表明该芯片的检测限为10-8 mol/L, 重复性测试的RSD约为11.85%。大肠杆菌SERS测试实验表明, 该芯片可快速获得大肠杆菌的拉曼特征峰, 无需对样本进行标记或复杂的前处理。该柔性纸基SERS芯片结构简单、制作快速、成本低廉, 阵列结构可实现多参数的同时测量, 有望应用于致病菌的直接、快速检测。

To achieve rapid, low-cost, and high-throughput detection of bacteria, an array flexible paper-based Surface-Enhanced Raman Scattering(SERS) detection chip was designed and fabricated. First, silver sol was prepared, and then the isolation area and the array detection area were constructed on paper base using the hydrophobicity of laser printing and carbon powder. Given the hydrophobic property of carbon powder, silver sol droplets were confined to the detection area. At a certain temperature, the silver sol dries naturally to form an array of active SERS detection chip. The paper-based SERS chip was characterized by Rhodamine 6G as probe molecule. The test results show that the detection limit of the chip is 10-8 mol/L, and the RSD of the repeatability test is about 11.85%. The SERS test of Escherichia coli(E. coli) shows that the chip can quickly obtain the Raman characteristic peaks of E. coli without labeling or complicated pretreatment. The advantages of the flexible paper-based SERS chip include simple structure, quick fabrication, and low cost. The array structure can realize simultaneous measurement of multiple parameters, and is expected to be used for direct and rapid detection of pathogenic bacteria.