光学技术, 2020, 46 (1): 41, 网络出版: 2020-04-13
细胞发酵罐显微光电检测系统显微镜头光学设计
Optical design of microscope for photodetection system of cell fermenter
光电检测 光学设计 卡塞格林系统 李斯特物镜 photoelectric detection optical design cassegrain system lister objective lens
摘要
提出一种在发酵罐外部架设显微系统进行非接触式细胞观测的光电检测方案。现有倒置生物显微镜工作距不足, 并且其自身光源发散角大、能量低不足以照亮细胞, 而工作距足够的工业显微镜分辨率最小只能达到10μm, 所培育的动植物细胞大小为10~20μm, 无法获得清晰的细胞图像。为满足系统指标, 设计了一种非接触式、折反射结合的长工作距可变焦光电检测系统。系统采用卡塞格林系统和李斯特物镜结合的设计思路, 解决了折射式结构像差大的缺点。光学系统放大倍率为3~5倍, 工作距为40mm, 物方数值孔径0.18~0.3, 分辨率可达到1.17~1.96μm, 其轴上点在CCD截止频率133lp/mm处均大于0.1, 大于人眼极限分辨阈值0.05。
Abstract
A method of photoelectric detection for non-contact cell observation is proposed. The existing inverted biological microscope has insufficient working distance and its own light source with large divergence angle and low energy is not enough to illuminate cells, the resolution of industrial microscopes with sufficient working distance is as low as 10 microns, the size of the plant and cultivated animal cells is 10~20 μm, and a clear cell image can not be obtained by existing inverted biological microscope. In order to meet the system specifications, a non-contact, anti-reflection combined long working distance zoom photodetection system is designed. The system adopts the design idea of combining Cassegrain system and Lister objective lens to solve the shortcomings of large refractive aberration of refractive structure. The magnification rate of the optical system is 3~5 times, the working distance is 40mm, the object numerical aperture is 0.18-0.3, the resolution can reach 1.17~1.96 microns, the axial point at the CCD cut-off frequency of 133lp/mm is greater than 0.1, greater than the human eye limit resolution threshold of 0.05.
窦伟, 黄浩, 李琦, 刘永志. 细胞发酵罐显微光电检测系统显微镜头光学设计[J]. 光学技术, 2020, 46(1): 41. DOU Wei, HUANG Hao, LI Qi, LIU Yongzhi. Optical design of microscope for photodetection system of cell fermenter[J]. Optical Technique, 2020, 46(1): 41.