中国激光, 2020, 47 (2): 0207029, 网络出版: 2020-02-21
β-淀粉样蛋白斑块的无标记成像及光动力降解 下载: 1238次
Label-Free Imaging of β-Amyloid Plaques and Photodynamic Degradation
医用光学 无标记成像 非线性光学成像 光动力治疗 阿尔兹海默症 纳米脂质体 medical optics label-free imaging nonlinear optical imaging photodynamic therapy Alzheimer''s disease nanoliposomes
摘要
由β-淀粉样蛋白(Aβ)生成的Aβ斑块是阿尔兹海默症(AD)产生的重要病理特征之一。在阿尔兹海默症治疗的相关研究中,实现对Aβ斑块的有效检测和降解是需要解决的重要科学问题。由于Aβ单体聚集成斑块后具有较强的自体荧光,因此本研究利用非线性光学成像方法实现对Aβ斑块的无标记成像,同时利用光敏剂的光动力效应对Aβ斑块进行降解。比较了不同浓度下光敏剂对Aβ斑块的降解效果,制备了相应的脂质体并成功地将其用于对Aβ斑块的降解。探讨了无标记光学成像及光动力疗法在阿尔兹海默症研究中的潜在应用价值,为优化阿尔兹海默症的诊断和治疗提供了新的途径。
Abstract
Formation of β-Amyloid (Aβ) plaques is one of the most significant pathological features of Alzheimer’s disease (AD). Detection and degradation of Aβ plaques are crucial in AD treatment. When Aβ monomers aggregate to form plaques, they produce strong autofluorescence. In this study, we investigate label-free imaging of Aβ plaques by using the nonlinear optical imaging method and study their degradation via photodynamic effect of the photosensitizer. Moreover, we study the relationship between photosensitizers with different concentrations and degradation effect of Aβ plaques. Corresponding liposomes are prepared and they are applied to the degradation of Aβ plaques. The potential applications of label-free optical imaging and photodynamic therapy in AD research are discussed, and a new way for optimizing AD diagnosis and treatment is provided.
黄燕霞, 许皓, 栾萍, OhulchanskyyTymishY, 屈军乐. β-淀粉样蛋白斑块的无标记成像及光动力降解[J]. 中国激光, 2020, 47(2): 0207029. Huang Yanxia, Xu Hao, Luan Ping, Ohulchanskyy Tymish Y, Qu Junle. Label-Free Imaging of β-Amyloid Plaques and Photodynamic Degradation[J]. Chinese Journal of Lasers, 2020, 47(2): 0207029.