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光片荧光显微成像技术及应用进展 (封面文章) (特邀综述)

Progress in Light-Sheet Fluorescence Microscopy and Applications (Cover Paper) (Invited)

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摘要

生物医学研究的发展对光学显微成像的性能,如空间分辨率、成像速度、多维度信息、成像质量等提出了更高的要求。光片荧光显微采用一个薄片光从侧面激发样品,在正交方向探测成像,具有快速三维层析成像和对样品光漂白和光毒性小的优点,是活体生物样品长时间显微观测的理想工具。本文介绍了光片荧光显微成像技术的基本原理及其主要特点;综述了光片荧光显微面临的主要技术问题,以及为解决这些问题而发展出的新原理、新思路和新方法;例举了光片荧光显微成像技术在细胞生物学、发育生物学和神经科学等领域的应用;讨论了该技术的发展趋势及前景。该研究旨在帮助研究者系统了解光片荧光显微成像技术的基本知识、最新研究发展趋势和潜在应用,为该领域科学研究提供参考。

Abstract

With the development of biomedical research, more advanced and comprehensive optical microscopy are required to enhance the imaging performances such as the spatial resolution, the imaging speed, the multi-dimensional information, the imaging quality, etc. Light-sheet fluorescence microscopy (LSFM), illuminating the specimen with a thin light sheet from the side and obtaining the optical sectioned image orthogonally, provides an ideal tool for long-term observation of live biological specimens due to its unique features of fast volumetric imaging speed and low photobleaching and phototoxicity to samples. In this paper, we first give an introduction to the principle and properties of LSFM. Then we address the key issues existing in LSFM and the solutions based on new principles, ideas and techniques. Some applications of LSFM in cell biology, developmental biology and neurosciences are exampled, and the development trends and prospect of LSFM are discussed. The purpose of the paper is to help researchers systematically understand the principle, the state-of-the-art techniques, and the potential applications of LSFM, and provide some references for research in this field.

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中图分类号:Q63

DOI:10.3788/LOP57.100001

所属栏目:综述

基金项目:国家自然科学基金;

收稿日期:2020-03-13

修改稿日期:2020-04-03

网络出版日期:2020-05-01

作者单位    点击查看

于湘华:中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119
刘超:中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119中国科学院大学, 北京 100049
柏晨:中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119
杨延龙:中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119
彭彤:中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119
但旦:中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119
闵俊伟:中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119
姚保利:中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119中国科学院大学, 北京 100049

联系人作者:姚保利(yaobl@opt.ac.cn)

备注:国家自然科学基金;

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引用该论文

Yu Xianghua,Liu Chao,Bai Chen,Yang Yanlong,Peng Tong,Dan Dan,Min Junwei,Yao Baoli. Progress in Light-Sheet Fluorescence Microscopy and Applications[J]. Laser & Optoelectronics Progress, 2020, 57(10): 100001

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