中国激光, 2020, 47 (5): 0509001, 网络出版: 2020-05-12
白光衍射相位像光晕效应消除方法 下载: 1109次
Elimination Method of Halo Artifacts in White-Light Diffraction Phase Microscopy
全息 白光衍射相位成像 相位像 光晕效应 希尔伯特变换 holography white-light diffraction phase imaging phase image halo effect Hilbert transform
摘要
白光扩展光源的低空间相干性,使得相位像不可避免地会遭受光晕效应的影响。基于此,提出了一种基于希尔伯特变换的快速消除光晕效应的方法。该方法不需要样品的任何先验知识,只需对测量相位像的导数作希尔伯特变换,并将变换后得到的图像和原测量图像中正确的高频数据和低频数据准确混合,即可有效地消除光晕效应,并保留白光相位成像系统的高分辨率。将所提方法应用于实验获取的白光衍射相位像数据,包括标准聚苯乙烯微球数据和活体血红细胞数据。实验结果表明,所提方法可以快速有效地消除白光衍射相位像的光晕效应。
Abstract
The phase image is inevitably affected by the halo effect because of the low spatial coherence of the extended white light source. Therefore, we propose a novel method of eliminating the halo effect based on the Hilbert transform in this study. This method does not require any prior knowledge about the measured sample and only needs Hilbert transform for obtaining the derivative of the measured phase image. Subsequently, the corrected low-frequency data and high-frequency data obtained from the acquired image after applying the Hilbert transform and from the original measured image, respectively, are carefully mixed. This method effectively eliminates the halo effects and preserves the high resolution of the white-light phase imaging system. The phase images obtained via white-light diffraction phase microscopy, including those of the standard polystyrene microspheres and the living red blood cells, are processed by applying the proposed method. The experimental results demonstrate that the proposed method can effectively and rapidly eliminate the halo effects associated with the white-light diffraction phase image.
丁海宁, 朱苗苗, 马利红, 李勇. 白光衍射相位像光晕效应消除方法[J]. 中国激光, 2020, 47(5): 0509001. Haining Ding, Miaomiao Zhu, Lihong Ma, Yong Li. Elimination Method of Halo Artifacts in White-Light Diffraction Phase Microscopy[J]. Chinese Journal of Lasers, 2020, 47(5): 0509001.