首页 > 论文 > 中国激光 > 46卷 > 9期(pp:907003--1)

基于光学相干层析成像技术的肿瘤细胞侵袭成像

Tumor Cell Invasion Imaging Based on Optical Coherence Tomography

  • 摘要
  • 论文信息
  • 参考文献
  • 被引情况
  • PDF全文
分享:

摘要

构建合适的肿瘤细胞侵袭模型、开发肿瘤细胞侵袭的定量监测方法一直是癌症研究的热点。构建了厚度超过1 mm的三维肿瘤体外侵袭模型,利用搭建的超宽带谱域光学相干层析成像系统,检测了细胞的迁移和侵袭动态,从细胞迁移距离变化和基质材料分解两方面来表征肿瘤细胞的体外侵袭过程;通过光学相干层析成像散射界面的峰值变化来定量检测肿瘤细胞的迁移距离,结合三维图像量化基质材料的表面曲度、厚度、整体体积变化来表征肿瘤细胞侵袭过程的基质材料分解与变形信息。结果表明:光学相干层析成像技术检测到的肿瘤细胞侵袭引起的细胞团簇位置变化、基质材料形态改变与苏木精-伊红染色切片、激光共聚焦结果相匹配,验证了光学相干层析成像技术检测肿瘤细胞侵袭的可行性;通过设计不同营养梯度、不同pH微环境下的三维肿瘤模型,利用搭建的光学相干层析成像系统,准确地量化了不同时间、不同体外微环境下肿瘤细胞的迁移距离、基质材料表面曲度、厚度和整体体积变化。与苏木精-伊红染色、激光共聚焦方法相比,所提方法可以连续监测肿瘤细胞的侵袭过程,更全面地反映肿瘤细胞迁移和侵袭的机理。

Abstract

Cancer research has increasingly focused on developing appropriate tumor cell invasion models and developing a quantitative method to monitor tumor cell invasion. In this study, a three-dimensional tumor invasive model with >1 mm thickness is constructed. An ultra-wideband spectral domain optical coherence tomography system is used to detect cell migration and invasion dynamics, and the in vitro invasion process of tumor cells is characterized by the change of cell migration distance and matrix material decomposition. The quantitative detection of tumor cell migration distance based on peak change of the optical coherence tomography scattering interface is combined with three-dimensional images to quantify the matrix surface curvature, thickness, and overall volume change, thereby realizing the characterization of the matrix material decomposition and deformation information during tumor cell invasion. The changes of cell cluster positions caused by tumor cell invasion and morphological changes of matrix materials are matched with hematoxylin-eosin staining sections and laser confocal results, which verifies the feasibility of optical coherence tomography for detecting tumor-cell invasion. Three-dimensional tumor models under different nutrient gradients and different pH microenvironments are utilized. The established optical coherence tomography system accurately quantifies the migration distance of tumor cells and surface curvature and overall volume change of matrix materials at different time and in vitro microenvironments. Compared with hematoxylin-eosin staining and the laser confocal imaging method, the proposed optical coherence tomography-based method enables the continuous monitoring of the invasion process of tumor cells, thereby providing a more comprehensive view of tumor-cell migration and invasion mechanisms.

Newport宣传-MKS新实验室计划
补充资料

DOI:10.3788/CJL201946.0907003

所属栏目:生物医学光子学与激光医学

基金项目:国家重点研发计划、国家自然科学基金;

收稿日期:2019-02-14

修改稿日期:2019-05-12

网络出版日期:2019-09-01

作者单位    点击查看

斯培剑:杭州电子科技大学自动化学院, 浙江 杭州 310018
王玲:杭州电子科技大学自动化学院, 浙江 杭州 310018浙江省医学信息与生物三维打印重点实验室, 浙江 杭州 310018
徐铭恩:杭州电子科技大学自动化学院, 浙江 杭州 310018浙江省医学信息与生物三维打印重点实验室, 浙江 杭州 310018

联系人作者:王玲, 徐铭恩(lingw@hdu.edu.cn, xumingen@hdu.edu.cn)

备注:国家重点研发计划、国家自然科学基金;

【1】Mayor R and Etienne-Manneville S. The front and rear of collective cell migration. Nature Reviews Molecular Cell Biology. 17(2), 97-109(2016).

【2】Zhu J R, Liang L, Jiao Y et al. Enhanced invasion of metastatic cancer cells via extracellular matrix interface. PLoS One. 10(2), (2015).

【3】Vicente-Manzanares M and Horwitz A R. Cell migration: an overview. 769, 1-24(2011).

【4】Marshall J. Transwell? invasion assays. 769, 97-110(2011).

【5】Kong X Q, Dong B L, Zhang N et al. A unique red-emitting two-photon fluorescent probe with tumor-specificity for imaging in living cells and tissues. Talanta. 174, 357-364(2017).

【6】Lü Y, Liu M, Zhang Y et al. Cancer cell membrane-biomimetic nanoprobes with two-photon excitation and near-infrared emission for intravital tumor fluorescence imaging. ACS Nano. 12(2), 1350-1358(2018).

【7】Kilin V, Mas C, Constant S et al. Health state dependent multiphoton induced autofluorescence in human 3D in vitro lung cancer model. Scientific Reports. 7, (2017).

【8】Lin H X, Zuo N, Zhuo S M et al. Application of multiphoton microscopy in disease diagnosis. Chinese Journal of Lasers. 45(2), (2018).
林宏心, 左宁, 卓双木 等. 多光子显微技术在医学诊断中的应用. 中国激光. 45(2), (2018).

【9】W?ldchen S, Lehmann J, Klein T et al. Light-induced cell damage in live-cell super-resolution microscopy. Scientific Reports. 5, (2015).

【10】Yang Y L, Zong W J, Wu R L et al. Light-sheet fluorescence microscopy. Acta Optica Sinica. 37(3), (2017).
杨豫龙, 宗伟建, 吴润龙 等. 光片荧光显微成像. 光学学报. 37(3), (2017).

【11】Liu L M and Qin H. Multiscale-photoacoustic microscopy for imaging cancer cells and solid tumors. Acta Laser Biology Sinica. 26(2), 116-120(2017).
刘丽鸣, 覃欢. 多尺度的广生显微镜用于癌细胞及实体瘤成像. 激光生物学报. 26(2), 116-120(2017).

【12】Olafsson R, Bauer D R, Montilla L G et al. Real-time, contrast enhanced photoacoustic imaging of cancer in a mouse window chamber. Optics Express. 18(18), 18625-18632(2010).

【13】Ragnar O, Bauer D R, Montilla L G et al. Real-time, contrast enhanced photoacoustic imaging of cancer in a mouse window chamber. Optics Express. 18(18), 18625-18632(2010).

【14】Zhang H F, Maslov K, Stoica G et al. Functional photoacoustic microscopy for high-resolution and noninvasive in vivo imaging. Nature Biotechnology. 24(7), 848-851(2006).

【15】Chen C J, Yang S H and Xing D. Progress and application of photoacoustic microscopy technique. Chinese Journal of Lasers. 45(3), (2018).
陈重江, 杨思华, 邢达. 光声显微成像技术研究进展及其应用. 中国激光. 45(3), (2018).

【16】Liang X, Graf B W and Boppart S A. Imaging engineered tissues using structural and functional optical coherence tomography. Journal of Biophotonics. 2(11), 643-655(2009).

【17】Huang D, Swanson E A, Lin C P et al. Optical coherence tomography. Science. 254(5035), 1178-1181(1991).

【18】Yang Y, Sule-Suso J et al. Use of OCT to study tumor cell growth in 3D membranes. Proceedings of SPIE. 5486, 154-160(2004).

【19】Tan W, Oldenburg A L, Norman J J et al. Optical coherence tomography of cell dynamics in three-dimensional tissue models. Optics Express. 14(16), 7159-7171(2006).

【20】Rey S M. Pova ay B, Hofer B, et al. Three- and four-dimensional visualization of cell migration using optical coherence tomography . Journal of Biophotonics. 2(6/7), 370-379(2009).

【21】Kuo C, Chan H, Chou H et al. Swept source optical coherence tomography for radiation-enhanced hepatocellular carcinoma cell invasion imaging. Physics in Medicine and Biology. 54(13), 4289-4297(2009).

【22】Chu K K, Kusek M E, Liu L B et al. Illuminating dynamic neutrophil trans-epithelial migration with micro-optical coherence tomography. Scientific Reports. 7, (2017).

【23】Zhang H, Chen M H, Li Z Y et al. The measurement of the concentration of glucose solution by using optical low coherence interferometry. Optical Technique. 44(3), 287-290(2018).
张浩, 陈明惠, 李振洋 等. 测量葡萄糖浓度的光学低相干干涉法. 光学技术. 44(3), 287-290(2018).

【24】Tan Z H, Feng Y P and Wang Z. Advances in measurement of optical central thickness by low coherence interferometry. Imaging Science and Photochemistry. 34(1), 5-14(2016).
檀泽浩, 冯云鹏, 王钟. 基于低相干干涉的透镜厚度测量及生物影像研究进展. 影像科学与光化学. 34(1), 5-14(2016).

【25】Wang Z B, Shi G H, He Y et al. Application of optical coherence tomography to distance measurement of optical surface. Optics and Precision Engineering. 20(7), 1469-1474(2012).
王志斌, 史国华, 何益 等. 光学相干层析技术在光学表面间距测量中的应用. 光学精密工程. 20(7), 1469-1474(2012).

【26】Xiao Z Y, Wu S L and Li H. Noninvasive measurement of epithelium thickness based on optical coherence tomography. Acta Laser Biology Sinica. 18(6), 810-814(2009).
肖郑颖, 吴淑莲, 李晖. 基于光学相干层析成像技术的小鼠表皮厚度的无损测量及分析. 激光生物学报. 18(6), 810-814(2009).

【27】Robertson C. Optical coherence tomography imaging of tissue engineered skin cultured under perfusion conditions. Sheffield: University of Sheffield. (2013).

【28】Sakai K, Kurokawa T, Furui Y et al. Invasion of carcinoma cells into reconstituted type I collagen gels: visual real-time analysis by time-lapse microscopy. BioScience Trends. 5(1), 10-16(2011).

【29】Zanotelli M R, Goldblatt Z E, Miller J P et al. Regulation of ATP utilization during metastatic cell migration by collagen architecture. Molecular Biology of the Cell. 29(1), 1-9(2018).

【30】Liu L, Duclos G, Sun B et al. Minimization of thermodynamic costs in cancer cell invasion. Proceedings of the National Academy of Sciences. 110(5), 1686-1691(2013).

【31】Levitz D, Hinds M T, Choudhury N et al. Quantitative characterization of developing collagen gels using optical coherence tomography. Journal of Biomedical Optics. 15(2), (2010).

【32】Zhou C F, Zhao S S, Sun Z Q et al. Acid conditions induces EMT of hepatocellular carcinoma HepG2 cells. Practical Oncology Journal. 31(3), 199-204(2017).
周春芳, 赵珊珊, 孙泽群 等. 酸性环境诱导肝癌HepG2细胞EMT. 实用肿瘤学杂志. 31(3), 199-204(2017).

【33】Huang Y B and Song F Z. The mechanism of pH regulation on tumor cell growth and metastasis. China Cancer. 21(6), 428-433(2012).
黄裕兵, 宋方洲. pH值调控肿瘤细胞生长转移的机制. 中国肿瘤. 21(6), 428-433(2012).

引用该论文

Peijian Si, Ling Wang, Ming''en Xu. Tumor Cell Invasion Imaging Based on Optical Coherence Tomography[J]. Chinese Journal of Lasers, 2019, 46(9): 0907003

斯培剑, 王玲, 徐铭恩. 基于光学相干层析成像技术的肿瘤细胞侵袭成像[J]. 中国激光, 2019, 46(9): 0907003

您的浏览器不支持PDF插件,请使用最新的(Chrome/Fire Fox等)浏览器.或者您还可以点击此处下载该论文PDF