激光与光电子学进展, 2020, 57 (12): 120001, 网络出版: 2020-06-03   

自适应光学在荧光显微镜中的应用 下载: 2668次封面文章特邀综述

Application of Adaptive Optics in Fluorescence Microscope
作者单位
1 西安电子科技大学物理与光电工程学院, 陕西 西安 710071
2 中国科学院西安光学精密机械研究所瞬态光学与光子技术国家重点实验室, 陕西 西安 710119
3 清华大学物理系低维量子物理国家重点实验室, 北京 100084
摘要
荧光显微镜因具有对样品损伤小、可进行特异性标记、适用于活体成像等优点,一直是生物医学研究中的主要手段。但光学系统自身缺陷、生物样品光学性质的不均匀性以及样品与显微镜浸润介质界面折射率的变化等导致了像差的产生,降低了成像的对比度和分辨率。自适应光学(AO)技术通过使用主动光学元件,如可变形镜、空间光调制器等,对畸变的波前(像差)进行校正,消除动态波前误差,恢复衍射受限性能。近年来,众多学者将AO系统与荧光显微镜相结合,以修正由样品不均匀性引起的像差,进而改善成像质量。介绍了AO技术的基本原理,综述了近年来AO技术在荧光显微镜成像中的应用,并对其未来发展趋势进行了展望。
Abstract
Fluorescence microscope has always been the main method in biomedical researches due to its advantages such as less damage to samples, specific labeling, and being suitable for in vivo imaging. However, the defects of optical system itself, the optical inhomogeneity of biological samples, and the change in the refractive index at the interface between the sample and the microscope's immersion medium have caused aberrations and reduced the imaging contrast and imaging resolution. Adaptive optics (AO) technology uses active optical components such as deformable mirrors and spatial light modulators to correct distorted wavefronts (aberrations), eliminate dynamic wavefront errors, and restore diffraction-limited performance. In recent years, many researchers have combined AO system with fluorescence microscope to correct the aberration caused by sample inhomogeneity and improve the imaging quality. In this paper, the basic principle of the AO technology is introduced, the applications of AO technology in fluorescence microscopic imaging in recent years are reviewed, and its future development trend is prospected.

刘立新, 张美玲, 吴兆青, 杨乾乾, 郜鹏, 薛平. 自适应光学在荧光显微镜中的应用[J]. 激光与光电子学进展, 2020, 57(12): 120001. Lixin Liu, Meiling Zhang, Zhaoqing Wu, Qianqian Yang, Peng Gao, Ping Xue. Application of Adaptive Optics in Fluorescence Microscope[J]. Laser & Optoelectronics Progress, 2020, 57(12): 120001.

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