Portable quantitative phase microscope for material metrology and biological imaging
Quantitative phase microscopy (QPM) has emerged as an important tool for material metrology and biological imaging. For broader adoption in those applications, we have proposed and demonstrated a new portable off-axis QPM method, which works in both transmission and reflection modes to meet different sample measurement requirements. The temporal and spatial sensitivities of our system, as quantified by optical path-length difference values, are 0.65 nm and 1.04 nm, respectively. To demonstrate its applicability for a wide range of applications, we deployed our system for profiling transistor gold electrode samples, observing red blood cell membrane fluctuations, imaging living cells flowing in a microfluidic chip, etc. Our portable QPM system has a low-cost design and involves a simple and robust phase-retrieval algorithm that we envision will allow for broader deployment at different environmental settings, including in resource-limited sites and integration with other metrology or imaging modalities.
Gang Luo：Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
Xin Shu：Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
Fuyang Qu：Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
Shuang Zhou：Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
Yi-Ping Ho：Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China；Centre for Novel Biomaterials, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
Ni Zhao：Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
Renjie Zhou：Department of Biomedical Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China；Shun Hing Institute of Advanced Engineering, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong SAR, China
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Mengxuan Niu, Gang Luo, Xin Shu, Fuyang Qu, Shuang Zhou, Yi-Ping Ho, Ni Zhao, and Renjie Zhou, "Portable quantitative phase microscope for material metrology and biological imaging," Photonics Research 8(7), 1253-1259 (2020)