将光热光学相干层析(PT-OCT)成像技术应用于肿瘤组织的三维成像。为了抑制噪声和增强图像对比度,提出互相关提取信号并计算平均幅值求解光程差成像的算法。在验证实验中,先后对琼脂样品和鼠耳模型进行成像。与普通OCT、基于相位差分算法的PT-OCT相比,本文方法可以很好地对鼠耳进行成像,能观察到更多的毛细血管,并且层析图也有更理想的成像效果。在肿瘤模型成像实验中,本文方法可以对4 mm×4 mm×3 mm的范围进行三维成像,并且可以从不同角度和不同的切面对三维PT-OCT图像进行观察,能够清楚地辨别其中的毛细血管分布。PT-OCT具有方便快速、非入侵、高分辨率、三维立体成像等优势,能为肿瘤临床诊断和治疗提供参考。

Photothermal optical coherence tomography (PT-OCT) imaging technology is applied to the three-dimensional imaging of tumor tissues. An algorithm, which uses the cross-correlation to extract the signal and calculates average amplitude for solving optical path difference imaging, is proposed to suppress noise and enhance image contrast. The agar sample and the mouse ear model are imaged successively in the validation experiment. Compared with the ordinary OCT and the PT-OCT based on the phase difference algorithm, the proposed method can be used for the mouse ear imaging with more details of capillaries, and the enhancement of the imaging effect of tomographic image can be obtained. In the experiment of imaging tumor model, the three-dimensional (3D) PT-OCT images are acquired from the excised tissues which are observed from different angles and different cut surfaces by the proposed method and the corresponding imaging range is 4 mm×4 mm×3 mm. The distribution of capillaries can be clearly identified. PT-OCT has the advantages of convenient, rapid, non-invasive, high-resolution, and 3D imaging, making it more applicable in clinical diagnosis and treatment of tumors.