为解决罗兰光栅光谱成像不易直接读取、平面场凹面光栅成像算法复杂等问题,设计了一种基于全息凹面光栅原理的用于特定蛋白分析仪生化检测的分光光度计。该结构以罗兰光栅为基底,采用双透镜作为过渡元件,将罗兰光栅的曲面成像过渡成平场光谱,同时采用光学设计软件Zemax对该结构进行模拟和优化。优化结果表明,分光光度计能够实现光谱平场化,工作波段为320~800 nm,最高分辨率可达0.5 nm,整体分辨率优于1.7 nm,可被光电二极管阵列直接读取,结构特性满足特定蛋白分析仪的测量要求。样机实验结果与模拟结果一致,仪器重复性、准确度、稳定性及线性度均达到行业标准。

In order to solve such problems as difficulty in directly reading the spectral imaging of Rowland grating and the complex imaging algorithm for flat-field concave gratings, we design a spectrophotometer based on principle of the holographic concave grating for biochemical detection of specific protein analyzers. This structure is based on the Rowland grating, and uses two lenses as the transition elements to transform the curved surface imaging of Rowland grating to the flat-field spectrum. Meanwhile, we simulate and optimize the structure with the optical design software Zemax. The optimization results show that the spectrophotometer whose working band is 320-800 nm with the highest resolution of 0.5 nm and the overall resolution of 1.7 nm, can be directly read by the photodiode array and can achieve plane spectrum. The structural characteristics meet the measurement requirements of specific protein analyzers. The experimental results of the prototype are consistent with the simulation results, and the repeatability, accuracy, stability, and linearity of the instrument are all up to the industry standards.