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A fast off-axis scanning subvoxel light-sheet microscope enables high-throughput image large-volume specimens at cellular resolution.

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The purpose of this study is to examine optical spatial frequency spectroscopy analysis (SFSA) combined with visible resonance Raman (VRR) spectroscopic method, for the first time, to discriminate human brain metastases of lung cancers adenocarcinoma (ADC) and squamous cell carcinoma (SCC) from normal tissues. A total of 31 label-free micrographic images of three types of brain

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In most coherent imaging modality, speckle noise is a major cause that blurs the boundary of tissues and degrades the image contrast. By utilizing the unique properties of supercontinuum (SC) generated by noise-like pulses (NLPs) and a simple multi-frame averaging technique, we achieved significant speckle reduction in spectral domain optical coherence tomography (SD-OCT). We q

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Diffuse optical spectroscopy is a relatively new, noninvasive and nonionizing technique for breast cancer diagnosis. In the present study, we have introduced a novel handheld diffuse optical breast scan (DOB-Scan) probe to measure optical properties of the breast in vivo and create functional and compositional images of the tissue. In addition, the probe gives more information

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The detection of early gastric cancer that often develops asymptomatically is crucial for improving patient survival. The photodynamic diagnosis (PDD) of gastric cancer using 5-aminolevulinic acid/protoporphyrin IX (5-ALA/PpIX) has been reported in several studies. However, the selectivity of PDD of gastric tumor is poor with often false-positive results that require the develo

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Laser speckle contrast imaging (LSCI) is an optical imaging method, which can monitor microvascular flow variation directly without addition of any ectogenous dye. All the existing laser speckle contrast analysis (LASCA) methods are a combination of spatial and temporal statistics. In this study, we have proposed a new method, Gaussian kernel laser speckle contrast analysis (gL

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As important components of air pollutant, volatile organic compounds (VOCs) can cause great harm to environment and human body. The concentration change of VOCs should be focused on in real-time environment monitoring system. In order to solve the problem of wavelength redundancy in full spectrum partial least squares (PLS) modeling for VOCs concentration analysis, a new method

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3D live imaging is important for better understanding of biological processes. To obtain biological dynamic process, high imaging speed is required. In order to improve speed in 3D live imaging, simultaneous imaging of multiple planes throughout a 3D volume has been proposed. However, a main disadvantage of this method is the cross-talk from neighboring imaging planes. In this

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This study aimed to explore the application of surface-enhanced Raman scattering (SERS) in the rapid diagnosis of gastric cancer. The SERS spectra of 68 serum samples from gastric cancer patients and healthy volunteers were acquired. The characteristic ratio method (CRM) and principal component analysis (PCA) were used to differentiate gastric cancer serum from normal serum. Co

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In illuminating tissues, a cylindrical diffuser (CD) has an advantage over regular laser sources due to its ability to illuminate a larger volume of the target tissue. This paper presents a co-registered large volume photoacoustic (PA) and ultrasonic (US) imaging for early endometrial cancer (EEC) detection using CD. It has the advantage that the US imaging system is outside th

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