光学学报, 2017, 37 (3): 0318010, 网络出版: 2017-03-08
多色单分子定位超分辨显微成像术 下载: 1016次
Multicolor Single-Molecule Localization Super-Resolution Microscopy
显微 荧光成像 单分子定位显微成像 多色成像 随机光学重建显微术 光敏定位显微术 microscopy fluorescence microscopy single molecule localization microscopy multi-color imaging stochastic optical reconstruction microscopy photo-activation localization microscopy
摘要
多色成像作为超分辨成像技术的重要延伸, 极大地增强了人们研究亚细胞结构定位与交互关系的能力, 从而有助于研究者深入理解细胞内复杂的生命现象与过程。基于单分子定位超分辨显微成像术(SMLM)工作原理的特殊性, 已实现了激发依赖、激活依赖、分光依赖等数种有特点的多色成像方法。介绍6种主要的多色单分子定位超分辨显微成像技术, 从分色能力、光谱窜扰、数据采集效率等角度分析了各方法的优缺点, 并讨论了与多色成像相关的细胞固定方法, 帮助研究人员根据自身实验需求选择合适可靠的多色成像手段研究相应的科学问题。
Abstract
Multicolor imaging is an important extension of super-resolution microscopy, which greatly enhances the investigation ability of the relationship between localization and interaction of sub-cellular structures. Therefore, it is beneficial for researchers to go deep into understanding of complicated biological phenomena and processes in cells. Based on the special working principle of single-molecule localization super-resolution microscopy (SMLM), several characteristic multicolor imaging technologies including excitation-dependent, activation-dependent, split-dependent multicolor SMLM and so on are accomplished. In this paper, we analyze the advantages and disadvantages among six main multicolor SMLM from the views of color separation ability, spectrum cross-talk and data collection efficiency. In addition, we discuss the cell fixation method in relation to the multicolor imaging. This review may be helpful for researchers to seek suitable and reliable multicolor imaging methods to study corresponding scientific problem according to their own experimental conditions.
潘雷霆, 胡芬, 张心正, 许京军. 多色单分子定位超分辨显微成像术[J]. 光学学报, 2017, 37(3): 0318010. Pan Leiting, Hu Fen, Zhang Xinzheng, Xu Jingjun. Multicolor Single-Molecule Localization Super-Resolution Microscopy[J]. Acta Optica Sinica, 2017, 37(3): 0318010.