Journal of Innovative Optical Health Sciences, 2019, 12 (5): 1940003, Published Online: Oct. 22, 2019  

Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications

Author Affiliations
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, P. R. China
Abstract
Inorganic quantum dots (QDs) have excellent optical properties, such as high fluorescence intensity, excellent photostability and tunable emission wavelength, etc., facilitating them to be used as labels and probes for bioimaging. In this study, CdSe@ZnS QDs are used as probes for Fluorescence lifetime imaging microscope (FLIM) and stimulated emission depletion (STED) nanoscopy imaging. The emission peak of CdSe@ZnS QDs centered at 526 nm with a narrow width of 19 nm and the photoluminescence quantum yield (PLQY) was 64%. The QDs presented excellent anti-photobleaching property which can be irradiated for 400 min by STED laser with 39.8mW. The lateral resolution of 42.0 nm is demonstrated for single QDs under STED laser (27.5mW) irradiation. Furthermore, the CdSe@ZnS QDs were for the first time used to successfully label the lysosomes of living HeLa cells and 81.5nm lateral resolution is obtained indicating the available super-resolution applications in living cells for inorganic QD probes. Meanwhile, Eca-109 cells labeled with the CdSe@ZnS QDs was observed with FLIM, and their fluorescence lifetime was around 3.1 ns, consistent with the in vitro value, suggesting that the QDs could act as a satisfactory probe in further FLIM-STED experiments.
References

[1] S. J. Sahl, W. Moerner, “Super-resolution fluorescence imaging with single molecules," Curr. Opin. Struct. Biol. 23, 778–787 (2013).

[2] E. Betzig, R. J. Chichester, “Single molecules observed by near-field scanning optical microscopy," Science 262, 1422–1425 (1993).

[3] S. W. Hell, N. S. Hell, J. Wichmann, “Breaking the diffraction resolution limit by stimulated emission: Stimulated-emission-depletion fluorescence microscopy," Opt. Lett. 19, 780–782 (1994).

[4] M. A. A. Neil, R. JusKaitis, T. Wilson, “Real time 3D fluorescence microscopy by two beam interference illumination," Opt. Commun. 153, 1–4 (1998).

[5] S. W. Hell, G. Donnert, J. Kelleretal, “Macromolecular-scale resolution in biological fluorescence microscopy," Proc. Natl. Acad. Sci. USA 103, 11440–11445 (2006).

[6] T. Grotjohann, I. Testa, M. Leutenegger, H. Bock, N. T. Urban, F. Lavoie-Cardinal, K. I. Willig, C. Eggeling, S. Jakobs, S. W. Hell, “Diffractionunlimited all-optical imaging and writing with a photochromic GFP," Nature 478, 204–208 (2011).

[7] M. Bossi, J. F€olling, M. Dyba, V. Westphal, S. W. Hell, “Breaking the diffraction resolution barrier in far-field microscopy by molecular optical bistability," New J. Phys. 8, 275(1–10) (2006).

[8] V. Westphal, S. W. Hell, “Nanoscale resolution in the focal plane of an optical microscope," Phys. Rev. Lett. 94, 143903(1–4) (2005).

[9] Y. Lin, K. Nienhaus, G. U. Nienhaus, “Nanoparticle probes for super-resolution fluorescence microscopy," ChemNanoMat. 4, 253–264 (2018).

[10] D. Jin, P. Xi, B. Wang, L. Zhang, J. Enderlein, A. M. van Oijen, Nanoparticles for super-resolution microscopy and single molecule tracking," Nat. Meth. 15, 415–423 (2018).

[11] L. Shang, P. Gao, H. Wang, R. Popescu, D. R. Gerthsend, G. U. Nienhaus, “Protein-based fluorescent nanoparticles for superresolution STED imaging of live cells," Chem. Sci. 8, 2396–2400 (2017).

[12] D. Weoll, C. Flors, “Super-resolution fluorescence imaging for materials science," Small Meth. 1, 1700191 (2017).

[13] D. Li, W. Qin, B. Xu, J. Qian, B. Z. Tang, “AIE nanoparticles with high stimulated emission depletion e±ciency and photobleaching resistance for long-term super-resolution bioimaging," Adv. Mater. 29(43), 1703643 (2017).

[14] G. Vicidomini, P. Bianchini, A. Diaspro, “STED super-resolved microscopy," Nat. Meth. 15, 173–182 (2018).

[15] Y. Liu, Y. Lu, X. Yang, X. Zheng, S. Wen, F. Wang, X. Vidal, J. Zhao, D. Liu, Z. Zhou, C. Ma, J. Zhou, J. A. Piper, P. Xi, D. Jin, “Amplified stimulated emission in upconversion nanoparticles for superresolution nanoscopy," Nature 545, 229–233 (2017).

[16] Q. Zhan, H. Liu, B. Wang, Q. Wu, R. Pu, C. Zhou, B. Huang, X. Peng, H. S Agren, S. He, “Achieving high-e±ciency emission depletion nanoscopy by employing cross relaxation in upconversion nanoparticles," Nat. Commun. 8, 1058 (2017).

[17] S. Ye, M. Zhao, J. Song, J. Qu, “Controllable emission bands and morphologies of high-quality CsPbX3 perovskite nanocrystals prepared in octane," Nano Res. 11, 4654–4663 (2018).

[18] S. Ye, W. Yan, M. Zhao, X. Peng, J. Song, J. Qu, “Low-saturation-intensity, high-photostability, and high-resolution STED nanoscopy assisted by CsPbBr3 quantum dots," Adv. Mater. 30, 1800167 (2018).

[19] S. E. Irvine, T. Staudt, E. Rittweger, J. Engelhardt, S. W. Hell, “Direct light-driven modulation of luminescence from Mn-doped ZnSe quantum dots," Angewandte Chemie 120, 2725–2728 (2008).

[20] G. Lemenager, E. D. Luca, Y. Sun, P. P. Pompa, “Super-resolution fluorescence imaging of biocompatible carbon dots," Nanoscale 6, 8617–8623 (2014).

[21] J. Hanne, H. J. Falk, F. Gorlitz, P. Hoyer, J. Engelhardt, S. J. Sahl, S. W. Hell, “STED nanoscopy with fluorescent quantum dots," Nat. Commun. 6, 7127 (2015).

[22] A. Pliss, X. Peng, L. Liu, A. Kuzmin, Y. Wang, J. Qu, Y. Li, P. N. Prasad, “Single cell assay for molecular diagnostics and medicine: Monitoring intracellular concentrations of macromolecules by two-photon fluorescence lifetime imaging," Theranostics 5, 919–930 (2015).

[23] S. Zhou, X. Peng, H. Xu, Y. Qin, D. Jiang, J. Qu, H. Y. Chen, “Fluorescence lifetime-resolved ionselective nanospheres for simultaneous imaging of calcium ion in mitochondria and lysosomes," Anal. Chem. 90, 7982–7988 (2018).

[24] A. Pliss, S. M. Levchenko, L. Liu, X. Peng, T. Y. Ohulchanskyy, I. Roy, A. N. Kuzmin, J. Qu, P. N. Prasad, “Cycles of protein condensation and discharge in nuclear organelles studied by fluorescence lifetime imaging, Nat. Commun. 10, 455 (2019).

Mengjie Zhao, Shuai Ye, Xiao Peng, Jun Song, Junle Qu. Green emitted CdSe@ZnS quantum dots for FLIM and STED imaging applications[J]. Journal of Innovative Optical Health Sciences, 2019, 12(5): 1940003.

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