以壳聚糖为原料, 经羧甲基化、纳米化与Ca2+、Zn2+复合制备出纳米羧甲基壳聚糖含钙、锌复合物, 采用红外光谱、X射线衍射光谱、扫描电镜等光学手段对样品进行结构表征, 考察了复合物的凝血、止血性能。结果表明: 纳米羧甲基壳聚糖结构中含有磷酸盐结构, Ca/Zn-纳米羧甲基壳聚糖结构中含有Ca2+与Zn2+, Ca2+和Zn2+与纳米羧甲基壳聚糖成功复合; 复合物的结晶性下降、溶解性提高、形貌均匀圆整, 且凝血、止血时间明显缩短; Ca-纳米羧甲基壳聚糖比Zn-纳米羧甲基壳聚糖结构更稳定, 凝血、止血效果更好。

With chitosan as raw material, Ca/Zn-nano carboxymethyl chitosan complexing was prepared through carboxymethylation, nanocrystallization and complexing with Ca2+, Zn2+. The structure was characterized by infrared spectroscopy, X-ray diffraction spectroscopy and scanning electron microscopy. The properties of coagulation and hemostatic were investigated. The results show that the polyphosphate structure is contained in nano carboxymethyl chitosan, as Ca2+ and Zn2+ are contained in Ca/Zn-nano carboxymethyl chitosan. Ca2+ and Zn2+ compound successfully with nano carboxymethyl chitosan. The composite has lower crystallization, higher solubility, more uniform round morphology and significant shorter coagulation and hemostasis time. Compared with Zn-nano carboxymethyl chitosan, Ca-nano carboxymethyl chitosan is possessed better structure and better properties of coagulation and hemostasis.