光学与光电技术, 2017, 15 (1): 81, 网络出版: 2017-02-23
多位相结构光照明显微重构技术仿真研究
Research on Multi-Phase Structure Illumination Microscopy Reconstruction Simulation Technology
成像系统 结构光 最小二乘法 超分辨率 图像重构 荧光显微镜 imaging system structured illumination least square method super resolution image reconstruction fluorescence microscope
摘要
为了观察细胞内部分子的结构特征和动态,提高光学显微镜分辨率成为生命科学研究的迫切要求。引入结构光照明的光学显微成像系统可以超越光学衍射极限,显著提高成像分辨率。在成像过程中,不可避免的噪声和相位误差降低了重构图像的质量,因此提出了一种基于最小二乘的多位相线性重构方法。在不同的噪声和相位误差条件下,对目标进行多次图像重构仿真模拟。结果显示:新方法能有效克服噪声和相位误差造成的重构图像失真,并具有较强的鲁棒性,在对比度、均方误差(MSE)以及视觉质量上有了较大的改善,信噪比(SNR)提高了约7dB、峰值信噪比(PSNR)提高了8 dB。
Abstract
In order to observe structural features and molecular dynamics in the cell, it becomes an urgent requirement of life science research to improve the resolution of optical microscopes. Optical microscopy imaging system with the structured light illumination beyond the optical diffraction limit can significantly improve image resolution. In the imaging process, the inevitable noise and phase error reduces the quality of the reconstructed image, and therefore a multi-phase linear reconstruction method based on least squares is proposed. Under different noise and phase error conditions, images were reconstructed by simulation several times. The results show that the new method is robust. The reconstructed image distortion caused by noise and phase errors is effectively overcome. The visual quality is greatly improved. The signal to noise ratio (SNR) is increased about 7 dB and peak signal to noise ratio (PSNR) is increased 8 dB.
朱琳, 郑伟波, 张涛. 多位相结构光照明显微重构技术仿真研究[J]. 光学与光电技术, 2017, 15(1): 81. ZHU Lin, ZHENG Wei-bo, ZHANG Tao. Research on Multi-Phase Structure Illumination Microscopy Reconstruction Simulation Technology[J]. OPTICS & OPTOELECTRONIC TECHNOLOGY, 2017, 15(1): 81.