中国激光, 2017, 44 (3): 0307001, 网络出版: 2017-03-08
锌酞菁体外光动力学疗法体外抗肿瘤细胞量效关系 下载: 596次
Dose-Effect Relationship of ZnPc-PDT on Tumor Cells In Vitro
医用光学 光动力疗法 锌酞菁 光敏剂 量效关系 单线态氧 medical optics photodynamic therapy ZnPc photosensitizer dose-effect relationship singlet oxygen
摘要
探讨光敏剂锌酞菁体外光动力疗法(ZnPc-PDT)抗肿瘤细胞的量效关系,为合理使用ZnPc提供参考。采用人脑胶质瘤细胞(U87 MG细胞)作为研究对象,用噻唑蓝(MTT)实验考察细胞存活率。采用单一变量的研究方法,分别考察了ZnPc浓度、激光功率密度、光照时间、体系氧含量和组织厚度等参量与U87 MG细胞存活率的关系。利用1,3-二苯基苯并呋喃(DPBF)和DCFH-DA活性氧探针分别检测了ZnPc-PDT过程中单线态氧的产量,并通过倒置显微镜观察经ZnPc-PDT治疗后的细胞形态和死亡细胞比例。结果表明,随着参量设置的变化,ZnPc-PDT对U87 MG细胞的杀伤力具有差异,通过调节参量,可达到较好的ZnPc-PDT治疗效果。
Abstract
We investigated the dose-effect relationship of photosensitizer ZnPc in photodynamic therapy (ZnPc-PDT) in killing the U87 MG cells and provided reference for rational administration of ZnPc. The proliferation of U87 MG cells was determined by MTT (thiazolyl blue tetrazolium bromide) tests. The effect of ZnPc concentration, laser power density, exposure time, oxygen content and tissue thickness on the proliferation was investigated. Then the production of singlet oxygen during ZnPc-PDT was determined by the DPBF probe and DCFH-DA reactive oxygen species assay kit. The cell morphology and the proportion of cell death was observed by an inverted microscope. The results show that U87 MG cell viability changes different parameters setting after ZnPc-PDT, and we can get a good ZnPc-PDT effect by adjusting the parameters.
杨曼, 邢力允, 高卫栋, 顾月清. 锌酞菁体外光动力学疗法体外抗肿瘤细胞量效关系[J]. 中国激光, 2017, 44(3): 0307001. Yang Man, Xing Liyun, Gao Weidong, Gu Yueqing. Dose-Effect Relationship of ZnPc-PDT on Tumor Cells In Vitro[J]. Chinese Journal of Lasers, 2017, 44(3): 0307001.