采用红外光谱法并结合二维相关红外光谱技术,对三七及其伪品姜黄、白术进行了快速无损的鉴别研究。在一维红外谱图上,三七及其伪品姜黄、白术中的黄酮成分、芳香成分和糖苷类成分的特征吸收峰均存在一定的差异。在1200~700 cm-1波数范围内,三七、姜黄均出现淀粉的特征峰,而白术没有显示淀粉的特征峰,根据各药材的红外光谱中淀粉的特征峰的相对强度,可判断三七中淀粉的相对含量要高于姜黄,而白术药材中不含淀粉。在二维红外谱图上,根据三者的自动峰的位置、数量和峰的相对强度的差异,可快速将三者鉴别出来。因此,常规红外光谱法和二维相关红外光谱技术相结合可实现三七的真伪鉴别。

Standard Panax notoginseng and its counterfeit Curcuma longa,Atractylodes macrocephala were identified fast and nondestructively by Fourier Transform Infrared Spectroscopy (FTIR) combined with two-dimensional correlation spectroscopy (2D-IR).Compared with conventional IR spectra of the samples,some certain differences were found in the characteristic peaks of flavonoids,aromatics and glycosides.In the range of 1 200-700 cm-1,it’s easy to find the fingerprint characteristic peaks of starch in the infrared spectra of Panax notoginseng and Curcuma longa,but Atractylodes macrocephala not.According to the relative intensity of starch characteristic peaks in the samples,it’s easy to find that the contents of starch in Panax notoginseng are higher than those in Curcuma longa,and Atractylodes macrocephala doesn’t contain starch.In the 2D-IR correlation spectra,the samples presented the differences in the position,number and relative intensity of autopeaks and correlation peak cluters.Consequently,Fourier Transform Infrared Spectroscopy (FTIR) combined with two-dimensional correlation spectroscopy (2D-IR) can be used to identified Panax notoginseng and its counterfeit Curcuma longa,Atractylodes macrocephala.