激光生物学报, 2017, 26 (3): 217, 网络出版: 2017-07-07  

ITO石英晶体电极上不同内皮细胞浓度的粘弹性研究及药物作用评估

Real Time Monitoring of Viscoelasticity of Different Human Umbilical Vein Endothelial Cell Concentrations on ITO Quartz Crystal Electrode and Evaluation of Cells-Drug Interactions Using Quartz Crystal Microbalance
作者单位
1 湖南农业大学生物科学技术学院, 湖南 长沙 410128
2 湖南农业大学细胞力学与生物传感研究所, 湖南 长沙 410128
摘要
用石英晶体微天平(quartz crystal microbalance, QCM)和活细胞成像技术实时监测人脐静脉内皮细胞(HUVEC)在ITO石英晶体电极上的动态粘附响应过程。在ITO晶体电极上加入不同浓度的HUVEC, 测定细胞在QCM上谐振频率以及耗散的实时变化。通过ITO电极与光学显微镜的联用, 监测了HUVEC在药物处理前后的动态变化过程。用细胞粘弹性指数(QCM的动态电阻变化与频移变化之比, CVI=ΔR/Δf)表征细胞的粘弹性变化, 同时通过活细胞成像技术的联用, 实时监测细胞的形态变化。结果表明: 细胞浓度为10万个/mL时, 细胞在ITO电极上铺展完全且粘弹性最大。抑制剂y-27632和激动剂凝血酶thrombin药物处理细胞前后, 在显微镜的实时监测下细胞形态变化不明显, 但CVI粘弹性指数变化较大, 说明QCM信号比光学信号更为敏感, 且在药物筛选方面有有很大的应用前景。
Abstract
In this study, the dynamic response of human umbilical vein endothelial cells (HUVECs) to ITO quartz crystal electrodes was monitored by quartz crystal microbalance (QCM) and live cell imaging. HUVECs with different cell concentrations were added to the ITO electrode. Real-time changes of QCM resonance frequency and dissipation were measured and compared with different concentrations of HUVEC. Through the combination of ITO electrode and optical microscope, the dynamic changes of HUVEC before and after drug treatment were also monitored. The Cell Viscoelastic Index (QCM ratio of dynamic resistance change to frequency shift, CVI=ΔR/ΔF) was used to characterize the cells’ viscoelasticity, at the same time the changes of cell morphological were real-time monitored by living cell imaging technology. The results showed that when the cell concentration was 10 000 cells/mL, the CVI was the maximum on the ITO electrode and HUVEC spread completely. The morphological changes of the cells were not obvious under the real-time monitoring of the microscope before and after drug treatment, but the changes of CVI was great . The results showed that the QCM signal was more sensitive than the optical signal.

鲍冬芹, 潘炜松, 谭成方, 沈海波, 周铁安. ITO石英晶体电极上不同内皮细胞浓度的粘弹性研究及药物作用评估[J]. 激光生物学报, 2017, 26(3): 217. BAO Dongqin, PAN Weisong, TAN Chengfang, SHEN Haibo, ZHOU Tie’an. Real Time Monitoring of Viscoelasticity of Different Human Umbilical Vein Endothelial Cell Concentrations on ITO Quartz Crystal Electrode and Evaluation of Cells-Drug Interactions Using Quartz Crystal Microbalance[J]. Acta Laser Biology Sinica, 2017, 26(3): 217.

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