激光生物学报, 2017, 26 (3): 255, 网络出版: 2017-07-07  

γ辐照对小麦种子延迟发光的影响

Effect of γ-rays Irradiation on Delayed Luminescence in Wheat Seed
作者单位
1 扬州大学生物科学与技术学院, 江苏 扬州 225009
2 江苏省农科院农业设施与装备研究所, 江苏 南京 210014
3 江苏省是里下河地区农业科学研究所, 江苏 扬州 225007
摘要
应用延迟发光的理论与方法, 研究了γ辐照对扬麦13、扬麦15两个品种小麦种子的影响。结果表明: 被测样品延迟发光的弛豫过程可以用非线性动力学方程Y^=b1+b2e-b3t1+b4t来进行描述, 该方程对测量结果拟合的相关系数达到了99%以上。当t=0时, Y^0 = b1 + b2 , 即样品的初始发光强度, 在光照条件一定时, 辐照剂量越高, 样品的初始发光强度就越低。F测验结果表明不同辐照剂量对小麦种子初始发光强度的影响达到了极显著的水平, 即受照剂量越高, 初始发光强度越低, 而两种供试小麦间的初始发光强度并无显著差异。结合处理种子苗期的生物学观察, 样品(即种子)的初始发光强度与幼苗株高具有一致的变化趋势, 即种子的初始发光强度越高, 幼苗株高也高。因此, 对辐射种子延迟发光过程的分析, 可能是了解γ辐照对作物种子影响的一种快速可靠的检测方法。
Abstract
Applying delayed luminescence analysis method, the effects of γ-rays irradiation on wheat seed (Yangmai 13 and Yangmai 15) was investigated. The relaxation of delayed luminescence could be expressed as the followed nonlinear equation, Y^=b1+b2e-b3t1+b4t, the correlation coefficent of measurement data with the equation above was over 99%. When t=0, Y^0 = b1 + b2 , under certain luminescence, the higher the radiation dose, the lower the initial luminescence intensity (Y^0)was. Further more, a significant negative correlativity between the radiation dose and the initial luminescence intensity was verified by the F test result, which was coincident between the both wheat varieties. The seedling height was coincident with the initial luminescence intensity of irradiated seed. Based on the data above, we could come to a conclusion that delayed luminescence technique would be useful for a deep understanding of the effeet of γ-rays on crops seed quality.

王泽港, 冯敏, 肖欢, 葛才林, 罗时石. γ辐照对小麦种子延迟发光的影响[J]. 激光生物学报, 2017, 26(3): 255. WANG Zegang, FENG Min, XIAO Huan, GE Cailin, LUO Shishi. Effect of γ-rays Irradiation on Delayed Luminescence in Wheat Seed[J]. Acta Laser Biology Sinica, 2017, 26(3): 255.

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