利用光镊拉曼光谱(LTRS)技术研究了不同环境因素对葡萄球菌黄素合成的影响。分别在温度范围为22~37 ℃、pH范围为4.5~8.5、葡萄糖质量浓度范围为0~10.0 g/L以及黄酮质量浓度为0~175 mg/L的条件下培养金黄色葡萄球菌, 利用拉曼光谱1523 cm-1特征峰强度表征细菌细胞内葡萄球菌黄素的相对含量。结果表明：在22~37 ℃范围内, 温度对葡萄球菌黄素合成的影响不大; 中性pH最有利于葡萄球菌黄素的合成; 葡萄糖在一定质量浓度范围内(0.2~5.0 g/L)可剂量依赖性地促进葡萄球菌黄素的合成, 黄酮也会剂量依赖性地抑制葡萄球菌黄素的合成。LTRS技术可作为快速可靠的葡萄球菌黄素定量检测方法以及葡萄球菌黄素合成抑制剂的有效筛选和评价手段。

Laser tweezers Raman spectroscopy (LTRS) is used to study the effects of various environmental factors on staphyloxanthin biosythesis. Staphylococcus aureus cells are cultivated at the temperature ranging from 22 ℃ to 37 ℃, pH from 4.5 to 8.5, glucose mass concentration from 0 g/L to 10.0 g/L, and flavone mass concentration from 0 mg/L to 175 mg/L. The intensity of Raman band at 1523 cm-1 is used to characterize the relative content of staphyloxanthin in bacterial cells. The experimental results show that the temperature ranging from 22 ℃ to 37 ℃ has no significant effect on staphyloxanthin biosynthesis, the neutral pH is most favorable for staphyloxanthin biosynthesis, and glucose in a certain mass concentration range (0.2-5.0 g/L) promotes staphyloxanthin synthesis in a dose-dependent manner whereas flavone inhibits staphyloxanthin synthesis in a dose-dependent manner. The LTRS technology can serve as a rapid and reliable method for quantitative determination of staphyloxanthin content as well as an effective approach to screening and evaluating inhibitors of staphyloxanthin biosynthesis.